Detailed information of TA system TA062131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 503829..504417
• Replicon: chromosome
• Accession: NZ_CP015030
• Organism: Mannheimia granulomatis strain B 234/94

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A011P4U0
• Locus tag: A4G16_RS02445
• Protein ID: WP_027074884.1
• Coordinates: 504121..504417 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: A4G16_RS02440
• Protein ID: WP_027074885.1
• Coordinates: 503829..504119 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A4G16_RS02410	499240..500286	-	1047	WP_165888546.1	conserved phage C-terminal domain-containing protein	-
A4G16_RS02415	500283..500963	-	681	WP_165888547.1	phage antirepressor KilAC domain-containing protein	-
A4G16_RS02420	501021..501245	-	225	WP_165888548.1	helix-turn-helix domain-containing protein	-
A4G16_RS02425	501370..502101	+	732	WP_165888549.1	helix-turn-helix transcriptional regulator	-
A4G16_RS02430	502105..502608	+	504	WP_165888550.1	DUF4365 domain-containing protein	-
A4G16_RS02435	502605..503714	+	1110	WP_165888551.1	hypothetical protein	-
A4G16_RS02440	503829..504119	-	291	WP_027074885.1	putative addiction module antidote protein	Antitoxin
A4G16_RS02445	504121..504417	-	297	WP_027074884.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A4G16_RS02450	505099..505422	-	324	WP_165888552.1	hypothetical protein	-
A4G16_RS02455	505619..505804	+	186	WP_165888553.1	hypothetical protein	-
A4G16_RS02460	505788..505985	+	198	WP_165888554.1	hypothetical protein	-
A4G16_RS02465	505988..506446	+	459	WP_165888555.1	single-stranded DNA-binding protein	-
A4G16_RS02470	506475..506834	+	360	WP_165888556.1	hypothetical protein	-
A4G16_RS02475	506905..507708	+	804	WP_165888557.1	DUF2303 family protein	-
A4G16_RS02480	507786..508070	+	285	WP_165888558.1	hypothetical protein	-
A4G16_RS02485	508081..508317	+	237	WP_165888559.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	469446..553537	84091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (4-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10945.81 Da
• Isoelectric Point: 10.3906

>T62131 WP_027074884.1 NZ_CP015030:c504417-504121 [Mannheimia granulomatis]
MIQIKSTTVFKEWLDDLKDLRARAKIQTRIKRLQLGNFGDVKAVGEGISELRITEGKGYRVYLKNQNGVIVILLCGGDKS
TQENDIKKAKALAKELGV

Nucleotide

• Length: 297 bp

>T62131 NZ_CP015030:c504417-504121 [Mannheimia granulomatis]
ATGATTCAGATTAAATCAACAACGGTTTTTAAAGAGTGGCTTGATGATTTAAAAGATTTACGAGCAAGGGCAAAAATTCA
AACTCGCATTAAGCGTTTGCAGCTGGGTAACTTTGGCGATGTAAAAGCAGTTGGAGAGGGTATTTCTGAATTGCGTATTA
CTGAGGGTAAAGGTTATCGGGTTTATCTAAAAAATCAGAATGGCGTTATTGTGATTTTATTGTGTGGTGGTGATAAATCG
ACACAAGAAAATGATATTAAAAAGGCGAAAGCATTAGCAAAAGAATTAGGAGTTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.23 Da
• Isoelectric Point: 4.6768

>AT62131 WP_027074885.1 NZ_CP015030:c504119-503829 [Mannheimia granulomatis]
MAELQDFDMAEHLRSEEEIQLYLNEVLKEDDVDLILSALGDIAKARNMSQLAREVGVSREGLYKAFSGKGNPTFSTVLKV
MKALNLTFEVKQAAHA

Nucleotide

• Length: 291 bp

>AT62131 NZ_CP015030:c504119-503829 [Mannheimia granulomatis]
ATGGCGGAACTACAAGATTTTGATATGGCGGAACATTTAAGAAGCGAAGAGGAAATTCAGCTTTACTTGAATGAAGTGTT
AAAAGAAGATGATGTGGACTTAATTCTTTCTGCATTGGGGGATATTGCCAAAGCACGAAATATGAGCCAGTTAGCGAGAG
AAGTGGGAGTAAGTCGAGAAGGGTTATATAAAGCCTTTTCCGGCAAAGGTAATCCAACATTTTCTACCGTGCTAAAAGTG
ATGAAGGCACTTAATCTTACTTTTGAAGTAAAGCAAGCTGCACACGCATAA

Structures

Toxin

• AlphaFold DB: A0A011P4U0