Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3849975..3850711 Replicon chromosome
Accession NZ_CP015025
Organism Klebsiella pneumoniae strain Kpn223

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag WM91_RS19090 Protein ID WP_032433360.1
Coordinates 3849975..3850457 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag WM91_RS19095 Protein ID WP_003026799.1
Coordinates 3850445..3850711 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WM91_RS19070 3846524..3847264 + 741 WP_050597964.1 molecular chaperone -
WM91_RS19075 3847268..3847846 + 579 WP_032432061.1 type 1 fimbrial protein -
WM91_RS19080 3847858..3848802 + 945 WP_077254249.1 fimbrial protein -
WM91_RS19085 3849015..3849614 + 600 WP_032432064.1 helix-turn-helix transcriptional regulator -
WM91_RS19090 3849975..3850457 - 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
WM91_RS19095 3850445..3850711 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
WM91_RS19100 3851024..3851587 - 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
WM91_RS19105 3851597..3852229 - 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
WM91_RS19110 3852247..3853251 - 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
WM91_RS19115 3853235..3853897 - 663 WP_032433366.1 dihydroxyacetone kinase subunit L -
WM91_RS19120 3853926..3855065 - 1140 WP_032433368.1 mannitol dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T62123 WP_032433360.1 NZ_CP015025:c3850457-3849975 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T62123 NZ_CP015025:c3850457-3849975 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT62123 WP_003026799.1 NZ_CP015025:c3850711-3850445 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT62123 NZ_CP015025:c3850711-3850445 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References