Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2450719..2451350 Replicon chromosome
Accession NZ_CP015023
Organism Escherichia coli strain SRCC 1675

Toxin (Protein)


Gene name hicA Uniprot ID Q8X5S6
Locus tag AR439_RS13315 Protein ID WP_001259386.1
Coordinates 2451075..2451350 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag AR439_RS13310 Protein ID WP_000593555.1
Coordinates 2450719..2451078 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AR439_RS13280 2445831..2446304 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
AR439_RS13285 2446301..2446582 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
AR439_RS13290 2446659..2448017 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
AR439_RS13295 2448010..2449518 + 1509 WP_001301773.1 carbohydrate kinase -
AR439_RS13300 2449508..2449777 + 270 WP_000933060.1 HPr family phosphocarrier protein -
AR439_RS13305 2449809..2450669 + 861 WP_001131788.1 class II aldolase -
AR439_RS13310 2450719..2451078 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
AR439_RS13315 2451075..2451350 - 276 WP_001259386.1 type II toxin-antitoxin system HicA family toxin Toxin
AR439_RS13320 2451423..2452547 - 1125 WP_001301659.1 ABC transporter permease -
AR439_RS13325 2452547..2455282 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
AR439_RS13330 2455279..2456346 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10165.92 Da        Isoelectric Point: 10.7076

>T62093 WP_001259386.1 NZ_CP015023:c2451350-2451075 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKLILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T62093 NZ_CP015023:c2451350-2451075 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGC
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT62093 WP_000593555.1 NZ_CP015023:c2451078-2450719 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT62093 NZ_CP015023:c2451078-2450719 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I9WXY7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References