Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 303537..304008 Replicon chromosome
Accession NZ_CP015023
Organism Escherichia coli strain SRCC 1675

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag AR439_RS02160 Protein ID WP_001303511.1
Coordinates 303730..304008 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag AR439_RS02155 Protein ID WP_001302048.1
Coordinates 303537..303728 (+) Length 64 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AR439_RS02110 298744..299328 - 585 WP_000206793.1 DUF551 domain-containing protein -
AR439_RS02115 299385..299780 - 396 WP_001118159.1 DUF977 family protein -
AR439_RS02120 299796..300565 - 770 Protein_308 DUF1627 domain-containing protein -
AR439_RS02130 300591..301331 - 741 WP_000788938.1 ATP-binding protein -
AR439_RS02135 301338..302300 - 963 WP_000095669.1 helix-turn-helix domain-containing protein -
AR439_RS02140 302323..302748 - 426 WP_000693943.1 Rha family transcriptional regulator -
AR439_RS02145 302745..303047 - 303 WP_000172738.1 transcriptional regulator -
AR439_RS31445 303145..303516 + 372 WP_001169686.1 hypothetical protein -
AR439_RS02155 303537..303728 + 192 WP_001302048.1 hypothetical protein Antitoxin
AR439_RS02160 303730..304008 + 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
AR439_RS02170 304361..304660 + 300 WP_001240334.1 hypothetical protein -
AR439_RS02175 304732..304950 + 219 WP_001171930.1 hypothetical protein -
AR439_RS02180 305519..305707 + 189 WP_000449192.1 cell division inhibition protein DicB -
AR439_RS02185 305704..305895 + 192 WP_001090200.1 DUF1482 family protein -
AR439_RS02190 305988..308459 + 2472 WP_062946113.1 exonuclease -
AR439_RS02195 308532..308783 + 252 WP_000005552.1 excisionase family protein -
AR439_RS32450 308818..309002 + 185 Protein_322 DUF3596 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 265716..318888 53172


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T62077 WP_001303511.1 NZ_CP015023:303730-304008 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T62077 NZ_CP015023:303730-304008 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT62077 WP_001302048.1 NZ_CP015023:303537-303728 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT62077 NZ_CP015023:303537-303728 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE

Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References