Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 84893..85418 Replicon plasmid pSRCC 1675
Accession NZ_CP015022
Organism Escherichia coli strain SRCC 1675

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag AR439_RS00480 Protein ID WP_001159868.1
Coordinates 85113..85418 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag AR439_RS00475 Protein ID WP_000813634.1
Coordinates 84893..85111 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AR439_RS31325 80176..80406 + 231 WP_010891288.1 hypothetical protein -
AR439_RS00440 80527..81267 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
AR439_RS00445 81552..82529 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
AR439_RS00455 82937..83137 - 201 WP_000708307.1 hypothetical protein -
AR439_RS00460 83134..83754 - 621 WP_001248529.1 hypothetical protein -
AR439_RS00465 83751..84434 - 684 WP_010891291.1 DNA-binding protein -
AR439_RS00475 84893..85111 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
AR439_RS00480 85113..85418 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
AR439_RS00485 85419..86225 + 807 WP_000016989.1 site-specific integrase -
AR439_RS00490 86948..88035 - 1088 Protein_92 IS3 family transposase -
AR439_RS28955 89331..89591 + 261 WP_071526583.1 RepB family plasmid replication initiator protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD 1..95170 95170


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T62075 WP_001159868.1 NZ_CP015022:85113-85418 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T62075 NZ_CP015022:85113-85418 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT62075 WP_000813634.1 NZ_CP015022:84893-85111 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT62075 NZ_CP015022:84893-85111 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References