Detailed information of TA system TA062074

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 36490..37049
• Replicon: plasmid pSRCC 1675
• Accession: NZ_CP015022
• Organism: Escherichia coli strain SRCC 1675

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: AR439_RS00215
• Protein ID: WP_000421248.1
• Coordinates: 36774..37049 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: AR439_RS00210
• Protein ID: WP_001178089.1
• Coordinates: 36490..36774 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AR439_RS00170	31682..32242	+	561	WP_000840472.1	fertility inhibition protein FinO	-
AR439_RS00175	32375..32587	+	213	WP_001302189.1	ANR family transcriptional regulator	-
AR439_RS00180	32832..33293	+	462	WP_001233853.1	thermonuclease family protein	-
AR439_RS00185	33339..33548	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
AR439_RS00190	33586..33924	+	339	WP_000766796.1	hypothetical protein	-
AR439_RS00195	34164..34418	+	255	WP_000083831.1	replication regulatory protein RepA	-
AR439_RS00200	34654..34728	+	75	WP_001370046.1	RepA leader peptide Tap	-
AR439_RS00205	34721..35578	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
AR439_RS00210	36490..36774	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
AR439_RS00215	36774..37049	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AR439_RS00220	37144..37350	+	207	WP_001105064.1	hypothetical protein	-
AR439_RS00225	37450..37665	-	216	Protein_46	transposase	-
AR439_RS28895	37709..38275	-	567	Protein_47	IS3-like element IS600 family transposase	-
AR439_RS00235	38275..38691	-	417	Protein_48	IS91 family transposase	-
AR439_RS00240	38691..38876	-	186	WP_001302179.1	hypothetical protein	-
AR439_RS00245	39053..41263	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
AR439_RS00250	41307..41696	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	toxB / espP / stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD	1..95170	95170

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T62074 WP_000421248.1 NZ_CP015022:36774-37049 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T62074 NZ_CP015022:36774-37049 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT62074 WP_001178089.1 NZ_CP015022:36490-36774 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT62074 NZ_CP015022:36490-36774 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8