Detailed information of TA system TA062073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 75108..75667
• Replicon: plasmid p28RC1
• Accession: NZ_CP015021
• Organism: Escherichia coli strain 28RC1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: ARC77_RS28870
• Protein ID: WP_000421248.1
• Coordinates: 75392..75667 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: ARC77_RS28865
• Protein ID: WP_001178089.1
• Coordinates: 75108..75392 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ARC77_RS28825	70301..70860	+	560	Protein_71	fertility inhibition protein FinO	-
ARC77_RS28830	70993..71205	+	213	WP_001302189.1	ANR family transcriptional regulator	-
ARC77_RS28835	71450..71911	+	462	WP_001233856.1	thermonuclease family protein	-
ARC77_RS28840	71957..72166	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
ARC77_RS28845	72204..72542	+	339	WP_000766796.1	hypothetical protein	-
ARC77_RS28850	72782..73036	+	255	WP_000083831.1	replication regulatory protein RepA	-
ARC77_RS28855	73272..73346	+	75	WP_001370046.1	RepA leader peptide Tap	-
ARC77_RS28860	73339..74196	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
ARC77_RS28865	75108..75392	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
ARC77_RS28870	75392..75667	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ARC77_RS28875	75762..75968	+	207	WP_001105064.1	hypothetical protein	-
ARC77_RS28880	76068..76283	-	216	Protein_82	transposase	-
ARC77_RS31335	76327..76893	-	567	Protein_83	IS3-like element IS600 family transposase	-
ARC77_RS28890	76893..77309	-	417	Protein_84	IS91 family transposase	-
ARC77_RS31340	77309..77494	-	186	WP_001302179.1	hypothetical protein	-
ARC77_RS28900	77671..79881	+	2211	WP_000592771.1	catalase/peroxidase KatP	-
ARC77_RS28905	79925..80314	+	390	WP_001172748.1	cytochrome b562 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	stcE / exeE / exeG / hlyC / hlyC / hlyA / hlyB / hlyD / toxB / toxB	1..81401	81401

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T62073 WP_000421248.1 NZ_CP015021:75392-75667 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T62073 NZ_CP015021:75392-75667 [Escherichia coli]
ATGGAACTGAAGTGGAGCAGTAAAGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT62073 WP_001178089.1 NZ_CP015021:75108-75392 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT62073 NZ_CP015021:75108-75392 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8