Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 31760..32285 Replicon plasmid p28RC1
Accession NZ_CP015021
Organism Escherichia coli strain 28RC1

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag ARC77_RS28630 Protein ID WP_001159868.1
Coordinates 31980..32285 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag ARC77_RS28625 Protein ID WP_000813634.1
Coordinates 31760..31978 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ARC77_RS32280 27043..27273 + 231 WP_010891288.1 hypothetical protein -
ARC77_RS28590 27394..28134 + 741 WP_001066920.1 tyrosine-type recombinase/integrase -
ARC77_RS28595 28419..29396 - 978 WP_000361615.1 RepB family plasmid replication initiator protein -
ARC77_RS28605 29804..30004 - 201 WP_000708307.1 hypothetical protein -
ARC77_RS28610 30001..30621 - 621 WP_001248529.1 hypothetical protein -
ARC77_RS28615 30618..31301 - 684 WP_010891291.1 DNA-binding protein -
ARC77_RS28625 31760..31978 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
ARC77_RS28630 31980..32285 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
ARC77_RS28635 32286..33092 + 807 WP_000016989.1 site-specific integrase -
ARC77_RS28650 35082..35444 + 363 Protein_34 RepB family plasmid replication initiator protein -
ARC77_RS28655 36032..37198 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - stcE / exeE / exeG / hlyC / hlyC / hlyA / hlyB / hlyD / toxB / toxB 1..81401 81401


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T62072 WP_001159868.1 NZ_CP015021:31980-32285 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T62072 NZ_CP015021:31980-32285 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT62072 WP_000813634.1 NZ_CP015021:31760-31978 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT62072 NZ_CP015021:31760-31978 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References