Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 5173643..5174438 Replicon chromosome
Accession NZ_CP015020
Organism Escherichia coli strain 28RC1

Toxin (Protein)


Gene name yeeV Uniprot ID B7UP43
Locus tag ARC77_RS26135 Protein ID WP_000854914.1
Coordinates 5174064..5174438 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A093DMQ4
Locus tag ARC77_RS26130 Protein ID WP_001280952.1
Coordinates 5173643..5174017 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ARC77_RS26090 5168991..5169896 + 906 WP_021497799.1 yjcZ-like family protein -
ARC77_RS26095 5169893..5170963 + 1071 WP_062946188.1 patatin-like phospholipase family protein -
ARC77_RS26105 5171303..5172121 + 819 WP_001506712.1 DUF945 domain-containing protein -
ARC77_RS26110 5172213..5172698 + 486 WP_001470565.1 antirestriction protein -
ARC77_RS26115 5172714..5173190 + 477 WP_001186714.1 RadC family protein -
ARC77_RS26120 5173259..5173480 + 222 WP_001220316.1 DUF987 domain-containing protein -
ARC77_RS26130 5173643..5174017 + 375 WP_001280952.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
ARC77_RS26135 5174064..5174438 + 375 WP_000854914.1 TA system toxin CbtA family protein Toxin
ARC77_RS26140 5174435..5174926 + 492 WP_000976857.1 hypothetical protein -
ARC77_RS26145 5174938..5175135 + 198 WP_000839282.1 DUF957 domain-containing protein -
ARC77_RS26150 5175220..5176062 + 843 WP_001280481.1 DUF4942 domain-containing protein -
ARC77_RS26160 5176533..5177471 + 939 WP_000351284.1 glyoxylate/hydroxypyruvate reductase GhrA -
ARC77_RS26165 5177526..5178263 + 738 WP_000283664.1 zinc-binding phosphatase -
ARC77_RS26170 5178287..5178841 + 555 WP_001001917.1 molecular chaperone YcdY -
ARC77_RS26175 5178943..5179434 + 492 WP_001301587.1 DUF1097 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-118)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14117.17 Da        Isoelectric Point: 7.7761

>T62069 WP_000854914.1 NZ_CP015020:5174064-5174438 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T62069 NZ_CP015020:5174064-5174438 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACCTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13719.50 Da        Isoelectric Point: 6.6249

>AT62069 WP_001280952.1 NZ_CP015020:5173643-5174017 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS

Download         Length: 375 bp

>AT62069 NZ_CP015020:5173643-5174017 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LXR5

Antitoxin

Source ID Structure
AlphaFold DB A0A093DMQ4

References