Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4313992..4314610 Replicon chromosome
Accession NZ_CP015020
Organism Escherichia coli strain 28RC1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ARC77_RS21980 Protein ID WP_001291435.1
Coordinates 4313992..4314210 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ARC77_RS21985 Protein ID WP_000344800.1
Coordinates 4314236..4314610 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ARC77_RS21945 4309279..4309851 + 573 WP_000779831.1 YbaY family lipoprotein -
ARC77_RS21950 4309884..4310195 - 312 WP_000409911.1 MGMT family protein -
ARC77_RS21960 4310574..4310927 + 354 WP_000878140.1 DUF1428 domain-containing protein -
ARC77_RS21965 4310969..4312519 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ARC77_RS21970 4312683..4313153 - 471 WP_000136192.1 YlaC family protein -
ARC77_RS21975 4313269..4313820 - 552 WP_000102553.1 maltose O-acetyltransferase -
ARC77_RS21980 4313992..4314210 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ARC77_RS21985 4314236..4314610 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ARC77_RS21990 4315156..4318305 - 3150 WP_001132452.1 multidrug efflux RND transporter permease subunit -
ARC77_RS21995 4318328..4319521 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T62063 WP_001291435.1 NZ_CP015020:c4314210-4313992 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T62063 NZ_CP015020:c4314210-4313992 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT62063 WP_000344800.1 NZ_CP015020:c4314610-4314236 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT62063 NZ_CP015020:c4314610-4314236 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACTCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References