Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2646662..2647293 Replicon chromosome
Accession NZ_CP015020
Organism Escherichia coli strain 28RC1

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag ARC77_RS13910 Protein ID WP_001259385.1
Coordinates 2647018..2647293 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag ARC77_RS13905 Protein ID WP_000593555.1
Coordinates 2646662..2647021 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ARC77_RS13875 2641774..2642247 + 474 WP_001161553.1 PTS sugar transporter subunit IIA -
ARC77_RS13880 2642244..2642525 + 282 WP_000084025.1 PTS sugar transporter subunit IIB -
ARC77_RS13885 2642602..2643960 + 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
ARC77_RS13890 2643953..2645461 + 1509 WP_001301773.1 carbohydrate kinase -
ARC77_RS13895 2645451..2645720 + 270 WP_000933060.1 HPr family phosphocarrier protein -
ARC77_RS13900 2645752..2646612 + 861 WP_001131788.1 class II aldolase -
ARC77_RS13905 2646662..2647021 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
ARC77_RS13910 2647018..2647293 - 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
ARC77_RS13915 2647366..2648490 - 1125 WP_001301659.1 ABC transporter permease -
ARC77_RS13920 2648490..2651225 - 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
ARC77_RS13925 2651222..2652289 - 1068 WP_000361458.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T62051 WP_001259385.1 NZ_CP015020:c2647293-2647018 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T62051 NZ_CP015020:c2647293-2647018 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT62051 WP_000593555.1 NZ_CP015020:c2647021-2646662 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT62051 NZ_CP015020:c2647021-2646662 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References