Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 545798..546436 Replicon chromosome
Accession NZ_CP015020
Organism Escherichia coli strain 28RC1

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag ARC77_RS02930 Protein ID WP_000813794.1
Coordinates 546260..546436 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag ARC77_RS02925 Protein ID WP_001270286.1
Coordinates 545798..546214 (-) Length 139 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ARC77_RS02905 540950..541891 - 942 WP_001251315.1 ABC transporter permease -
ARC77_RS02910 541892..542905 - 1014 WP_000220402.1 ABC transporter ATP-binding protein -
ARC77_RS02915 542923..544068 - 1146 WP_000047432.1 ABC transporter substrate-binding protein -
ARC77_RS02920 544313..545719 - 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
ARC77_RS02925 545798..546214 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
ARC77_RS02930 546260..546436 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
ARC77_RS02935 546658..546888 + 231 WP_000494244.1 YncJ family protein -
ARC77_RS02940 546980..548941 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
ARC77_RS02945 549014..549550 - 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
ARC77_RS02950 549642..550815 + 1174 Protein_562 BenE family transporter YdcO -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-57)

Antitoxin

(91-134)


Sequences


Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T62041 WP_000813794.1 NZ_CP015020:c546436-546260 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T62041 NZ_CP015020:c546436-546260 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT62041 WP_001270286.1 NZ_CP015020:c546214-545798 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT62041 NZ_CP015020:c546214-545798 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 6HPB


Antitoxin

Source ID Structure

References