Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 323568..324039 Replicon chromosome
Accession NZ_CP015020
Organism Escherichia coli strain 28RC1

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A0D7C2L1
Locus tag ARC77_RS01680 Protein ID WP_001303511.1
Coordinates 323568..323846 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID Q8XAD5
Locus tag ARC77_RS01685 Protein ID WP_001302048.1
Coordinates 323848..324039 (-) Length 64 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ARC77_RS32355 318575..318759 - 185 Protein_317 DUF3596 domain-containing protein -
ARC77_RS01645 318794..319045 - 252 WP_000005552.1 excisionase family protein -
ARC77_RS01650 319118..321589 - 2472 WP_000048458.1 exonuclease -
ARC77_RS01655 321682..321873 - 192 WP_001090200.1 DUF1482 family protein -
ARC77_RS01660 321870..322058 - 189 WP_000449192.1 cell division inhibition protein DicB -
ARC77_RS01665 322627..322845 - 219 WP_001171930.1 hypothetical protein -
ARC77_RS01670 322917..323216 - 300 WP_001240334.1 hypothetical protein -
ARC77_RS01680 323568..323846 - 279 WP_001303511.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
ARC77_RS01685 323848..324039 - 192 WP_001302048.1 hypothetical protein Antitoxin
ARC77_RS31385 324060..324431 - 372 WP_001169686.1 hypothetical protein -
ARC77_RS01695 324529..324831 + 303 WP_000172738.1 transcriptional regulator -
ARC77_RS01700 324828..325253 + 426 WP_000693943.1 Rha family transcriptional regulator -
ARC77_RS01705 325276..326238 + 963 WP_000095669.1 helix-turn-helix domain-containing protein -
ARC77_RS01710 326245..326985 + 741 WP_000788938.1 ATP-binding protein -
ARC77_RS01720 327011..327780 + 770 Protein_331 DUF1627 domain-containing protein -
ARC77_RS01725 327796..328155 + 360 WP_062946123.1 DUF977 family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - nleG7' 316408..416304 99896


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-91)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 93 a.a.        Molecular weight: 10661.25 Da        Isoelectric Point: 5.5647

>T62038 WP_001303511.1 NZ_CP015020:c323846-323568 [Escherichia coli]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Download         Length: 279 bp

>T62038 NZ_CP015020:c323846-323568 [Escherichia coli]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin


Download         Length: 64 a.a.        Molecular weight: 7463.47 Da        Isoelectric Point: 8.6828

>AT62038 WP_001302048.1 NZ_CP015020:c324039-323848 [Escherichia coli]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Download         Length: 192 bp

>AT62038 NZ_CP015020:c324039-323848 [Escherichia coli]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5CZF
PDB 5CW7
PDB 5CZE


Antitoxin

Source ID Structure
PDB 5CZF
PDB 3ZBE
PDB 5CW7
AlphaFold DB A0A829CTP0

References