Detailed information of TA system TA062029

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1282769..1283352
• Replicon: chromosome
• Accession: NZ_CP015018
• Organism: Polynucleobacter asymbioticus strain Tro8F10W22

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AOC10_RS06505
• Protein ID: WP_071540440.1
• Coordinates: 1282769..1283110 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AOC10_RS06510
• Protein ID: WP_071540441.1
• Coordinates: 1283110..1283352 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOC10_RS06485	1278529..1279386	+	858	WP_071540437.1	carboxylating nicotinate-nucleotide diphosphorylase	-
AOC10_RS06490	1279394..1280773	-	1380	WP_071540438.1	hypothetical protein	-
AOC10_RS06495	1280839..1282497	-	1659	WP_011902947.1	L-aspartate oxidase	-
AOC10_RS06505	1282769..1283110	-	342	WP_071540440.1	endoribonuclease MazF	Toxin
AOC10_RS06510	1283110..1283352	-	243	WP_071540441.1	PbsX family transcriptional regulator	Antitoxin
AOC10_RS06515	1283499..1286390	-	2892	WP_071540442.1	formate dehydrogenase subunit alpha	-
AOC10_RS06520	1286430..1288232	-	1803	WP_071540443.1	NAD(P)H-dependent oxidoreductase subunit E	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-108)

Antitoxin

• Antitoxin PemI-like (1-79)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12508.65 Da
• Isoelectric Point: 9.4702

>T62029 WP_071540440.1 NZ_CP015018:c1283110-1282769 [Polynucleobacter asymbioticus]
MVRAYVPEAGDIVWLECDPQAGREQAGRRPTVVLTPEAYNKKTNLMICCPLTTQIKGYPFEVLVAVDGVQSAILSDQVKS
LDWKIRKAKYKNKVSSEILGEVKAKAKSLLSIT

Nucleotide

• Length: 342 bp

>T62029 NZ_CP015018:c1283110-1282769 [Polynucleobacter asymbioticus]
ATGGTGAGAGCTTATGTGCCAGAAGCTGGTGATATTGTATGGCTGGAGTGTGATCCTCAAGCCGGAAGAGAGCAAGCTGG
TCGTCGACCTACTGTTGTGTTGACGCCAGAGGCCTATAACAAAAAAACTAATCTCATGATTTGTTGCCCATTAACAACCC
AGATTAAAGGTTATCCCTTTGAGGTTCTAGTTGCAGTGGATGGGGTTCAATCAGCGATCTTGTCTGATCAGGTTAAGTCC
TTGGATTGGAAAATAAGAAAAGCAAAATACAAAAATAAAGTGAGCTCAGAGATCCTTGGTGAAGTAAAAGCTAAGGCAAA
GTCTTTGCTCTCTATTACGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8784.29 Da
• Isoelectric Point: 8.6200

>AT62029 WP_071540441.1 NZ_CP015018:c1283352-1283110 [Polynucleobacter asymbioticus]
MQTMIKKWGNSPALRLNATVMKSAQLNIDQLVSVKVQKGRIVIEPIVKQEYPLEELLAGITAKNLHSEADFGGPVGNELL

Nucleotide

• Length: 243 bp

>AT62029 NZ_CP015018:c1283352-1283110 [Polynucleobacter asymbioticus]
ATGCAAACAATGATTAAAAAGTGGGGTAATAGCCCAGCTTTACGTTTAAATGCTACGGTAATGAAGTCTGCCCAATTGAA
TATTGATCAACTTGTCTCTGTAAAGGTTCAAAAAGGTCGCATCGTGATCGAGCCTATAGTCAAGCAGGAGTATCCACTCG
AAGAATTGTTGGCTGGTATTACTGCTAAGAATTTGCATAGCGAAGCAGACTTTGGTGGCCCCGTGGGTAATGAGCTTCTC
TAA