Detailed information of TA system TA062028

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 777451..778082
• Replicon: chromosome
• Accession: NZ_CP015018
• Organism: Polynucleobacter asymbioticus strain Tro8F10W22

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AOC10_RS04090
• Protein ID: WP_011902531.1
• Coordinates: 777684..778082 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: AOC10_RS04085
• Protein ID: WP_011902530.1
• Coordinates: 777451..777684 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AOC10_RS04070	772637..774256	-	1620	WP_011902527.1	NAD+ synthase	-
AOC10_RS04075	774294..774830	-	537	WP_011902528.1	inorganic diphosphatase	-
AOC10_RS04080	775003..777063	+	2061	WP_011902529.1	sodium-translocating pyrophosphatase	-
AOC10_RS04085	777451..777684	+	234	WP_011902530.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AOC10_RS04090	777684..778082	+	399	WP_011902531.1	type II toxin-antitoxin system VapC family toxin	Toxin
AOC10_RS04095	778150..779106	-	957	WP_011902532.1	thioredoxin-disulfide reductase	-
AOC10_RS04100	779146..781458	+	2313	WP_011902533.1	DNA translocase FtsK	-
AOC10_RS04105	781468..782151	+	684	WP_011902534.1	outer membrane lipoprotein carrier protein LolA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-123)

Antitoxin

• SpoVT-AbrB domain (7-50)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14965.45 Da
• Isoelectric Point: 9.3007

>T62028 WP_011902531.1 NZ_CP015018:777684-778082 [Polynucleobacter asymbioticus]
MLKYLLDTNIVIYVIKRKPLEVLKVFNKNANRMAISAITLAELIYGAEKSKQVEKNLNVIEDFISHLEVLPYDITATQHY
GQIKAFLEHSGKPIGVNDIHIAAHARSHGLTLVTNNMSEFKRVPNLALENWV

Nucleotide

• Length: 399 bp

>T62028 NZ_CP015018:777684-778082 [Polynucleobacter asymbioticus]
ATGCTGAAGTACTTATTGGACACAAATATTGTTATTTATGTCATAAAGCGAAAACCGCTAGAGGTGCTTAAAGTTTTCAA
CAAAAATGCTAATCGTATGGCTATATCAGCAATTACATTGGCAGAGCTAATTTATGGCGCCGAAAAAAGTAAGCAAGTAG
AAAAAAATCTTAATGTTATTGAAGACTTCATCAGCCATTTAGAGGTTTTACCGTATGACATTACCGCAACTCAGCATTAC
GGGCAAATTAAGGCTTTTTTAGAGCACTCAGGCAAGCCTATTGGAGTGAATGATATTCATATTGCAGCTCACGCCAGAAG
TCATGGCTTAACTTTGGTGACTAACAATATGTCTGAGTTTAAGAGGGTCCCAAACCTTGCCCTTGAAAACTGGGTTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8826.92 Da
• Isoelectric Point: 5.0959

>AT62028 WP_011902530.1 NZ_CP015018:777451-777684 [Polynucleobacter asymbioticus]
MAISTVFTNNRTQAVRLPAEMRLPENVRKVNVRSKGRERIIAPIENMWDNFFLGEAGVSDDFMNERGSQEQGDRESL

Nucleotide

• Length: 234 bp

>AT62028 NZ_CP015018:777451-777684 [Polynucleobacter asymbioticus]
ATGGCTATCTCAACAGTGTTCACCAATAACCGAACTCAGGCAGTGAGATTGCCTGCAGAAATGCGATTGCCTGAGAATGT
ACGCAAGGTTAACGTACGATCAAAAGGCCGTGAGCGCATCATTGCCCCTATAGAAAATATGTGGGACAACTTCTTCTTGG
GTGAAGCGGGTGTAAGTGACGACTTTATGAATGAAAGAGGCTCTCAGGAGCAAGGTGATAGAGAGTCCCTTTAA