Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1280097..1280680 Replicon chromosome
Accession NZ_CP015017
Organism Polynucleobacter asymbioticus strain MWH-RechtKol4

Toxin (Protein)


Gene name mazF Uniprot ID A0A1J0D5P7
Locus tag AOC25_RS06450 Protein ID WP_071539277.1
Coordinates 1280097..1280438 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1J0D5Q7
Locus tag AOC25_RS06455 Protein ID WP_071539278.1
Coordinates 1280438..1280680 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AOC25_RS06430 1275857..1276714 + 858 WP_071539273.1 carboxylating nicotinate-nucleotide diphosphorylase -
AOC25_RS06435 1276722..1278101 - 1380 WP_071539274.1 hypothetical protein -
AOC25_RS06440 1278167..1279825 - 1659 WP_071539275.1 L-aspartate oxidase -
AOC25_RS06445 1279861..1280061 - 201 WP_071539276.1 hypothetical protein -
AOC25_RS06450 1280097..1280438 - 342 WP_071539277.1 endoribonuclease MazF Toxin
AOC25_RS06455 1280438..1280680 - 243 WP_071539278.1 PbsX family transcriptional regulator Antitoxin
AOC25_RS06460 1280827..1283718 - 2892 WP_071539279.1 formate dehydrogenase subunit alpha -
AOC25_RS06465 1283757..1285559 - 1803 WP_071540124.1 NAD(P)H-dependent oxidoreductase subunit E -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-108)

Antitoxin

Antitoxin PemI-like

(1-79)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12594.76 Da        Isoelectric Point: 9.7231

>T62026 WP_071539277.1 NZ_CP015017:c1280438-1280097 [Polynucleobacter asymbioticus]
MVRAYVPEAGDIVWLEFDPQAGREQTGRRPAVVLTPEVYNKKTNLMICCPLTTQIKGYPFEVLVVVDGDQSAILSDQVKS
LDWKIRKAKYKNKVSSVILGEVKAKAKSLLSIT

Download         Length: 342 bp

>T62026 NZ_CP015017:c1280438-1280097 [Polynucleobacter asymbioticus]
ATGGTGAGAGCTTATGTGCCAGAAGCTGGTGATATTGTATGGCTGGAGTTTGATCCTCAAGCCGGAAGAGAGCAAACTGG
TCGTCGACCTGCTGTTGTGTTGACGCCAGAGGTCTATAACAAAAAAACTAATCTCATGATTTGTTGCCCATTAACAACCC
AGATTAAAGGTTATCCCTTTGAGGTTCTAGTTGTAGTGGATGGGGATCAATCAGCGATCTTGTCTGATCAGGTTAAGTCC
TTGGATTGGAAAATAAGAAAAGCAAAATACAAAAATAAAGTGAGCTCAGTGATCCTTGGTGAAGTAAAAGCTAAAGCAAA
GTCTTTGCTCTCTATTACGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8768.34 Da        Isoelectric Point: 9.7703

>AT62026 WP_071539278.1 NZ_CP015017:c1280680-1280438 [Polynucleobacter asymbioticus]
MQTMIKKWGNSPALRLNAAVMKSAQLNIDQLVSVKVQKGRIVIEPIVKQEYPLEELLAGITAKNLHSEADFGGPVGKELL

Download         Length: 243 bp

>AT62026 NZ_CP015017:c1280680-1280438 [Polynucleobacter asymbioticus]
ATGCAAACAATGATTAAAAAGTGGGGTAACAGCCCGGCTTTACGTTTAAATGCTGCGGTAATGAAGTCTGCCCAATTGAA
TATTGATCAACTTGTCTCTGTAAAGGTTCAAAAAGGTCGCATCGTGATCGAGCCTATAGTCAAGCAGGAGTATCCACTCG
AAGAATTGTTGGCTGGTATTACTGCTAAGAATTTGCATAGCGAAGCAGACTTTGGTGGCCCCGTGGGTAAAGAGCTTCTC
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1J0D5P7

Antitoxin

Source ID Structure
AlphaFold DB A0A1J0D5Q7

References