Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1261264..1261847 Replicon chromosome
Accession NZ_CP015016
Organism Polynucleobacter asymbioticus strain MWH-RechtKolB

Toxin (Protein)


Gene name mazF Uniprot ID A0A1J0D5P7
Locus tag A4F89_RS06365 Protein ID WP_071539277.1
Coordinates 1261264..1261605 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1J0D5Q7
Locus tag A4F89_RS06370 Protein ID WP_071539278.1
Coordinates 1261605..1261847 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A4F89_RS06345 1257024..1257881 + 858 WP_071539273.1 carboxylating nicotinate-nucleotide diphosphorylase -
A4F89_RS06350 1257889..1259268 - 1380 WP_071539274.1 hypothetical protein -
A4F89_RS06355 1259334..1260992 - 1659 WP_071539275.1 L-aspartate oxidase -
A4F89_RS06360 1261028..1261228 - 201 WP_071539276.1 hypothetical protein -
A4F89_RS06365 1261264..1261605 - 342 WP_071539277.1 endoribonuclease MazF Toxin
A4F89_RS06370 1261605..1261847 - 243 WP_071539278.1 PbsX family transcriptional regulator Antitoxin
A4F89_RS06375 1261994..1264885 - 2892 WP_071539279.1 formate dehydrogenase subunit alpha -
A4F89_RS06380 1264924..1266726 - 1803 WP_071539280.1 NAD(P)H-dependent oxidoreductase subunit E -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-108)

Antitoxin

Antitoxin PemI-like

(1-79)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12594.76 Da        Isoelectric Point: 9.7231

>T62025 WP_071539277.1 NZ_CP015016:c1261605-1261264 [Polynucleobacter asymbioticus]
MVRAYVPEAGDIVWLEFDPQAGREQTGRRPAVVLTPEVYNKKTNLMICCPLTTQIKGYPFEVLVVVDGDQSAILSDQVKS
LDWKIRKAKYKNKVSSVILGEVKAKAKSLLSIT

Download         Length: 342 bp

>T62025 NZ_CP015016:c1261605-1261264 [Polynucleobacter asymbioticus]
ATGGTGAGAGCTTATGTGCCAGAAGCTGGTGATATTGTATGGCTGGAGTTTGATCCTCAAGCCGGAAGAGAGCAAACTGG
TCGTCGACCTGCTGTTGTGTTGACGCCAGAGGTCTATAACAAAAAAACTAATCTCATGATTTGTTGCCCATTAACAACCC
AGATTAAAGGTTATCCCTTTGAGGTTCTAGTTGTAGTGGATGGGGATCAATCAGCGATCTTGTCTGATCAGGTTAAGTCC
TTGGATTGGAAAATAAGAAAAGCAAAATACAAAAATAAAGTGAGCTCAGTGATCCTTGGTGAAGTAAAAGCTAAAGCAAA
GTCTTTGCTCTCTATTACGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8768.34 Da        Isoelectric Point: 9.7703

>AT62025 WP_071539278.1 NZ_CP015016:c1261847-1261605 [Polynucleobacter asymbioticus]
MQTMIKKWGNSPALRLNAAVMKSAQLNIDQLVSVKVQKGRIVIEPIVKQEYPLEELLAGITAKNLHSEADFGGPVGKELL

Download         Length: 243 bp

>AT62025 NZ_CP015016:c1261847-1261605 [Polynucleobacter asymbioticus]
ATGCAAACAATGATTAAAAAGTGGGGTAACAGCCCGGCTTTACGTTTAAATGCTGCGGTAATGAAGTCTGCCCAATTGAA
TATTGATCAACTTGTCTCTGTAAAGGTTCAAAAAGGTCGCATCGTGATCGAGCCTATAGTCAAGCAGGAGTATCCACTCG
AAGAATTGTTGGCTGGTATTACTGCTAAGAATTTGCATAGCGAAGCAGACTTTGGTGGCCCCGTGGGTAAAGAGCTTCTC
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1J0D5P7

Antitoxin

Source ID Structure
AlphaFold DB A0A1J0D5Q7

References