Detailed information of TA system TA061997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 5402183..5402791
• Replicon: chromosome
• Accession: NZ_CP015003
• Organism: Pseudomonas aeruginosa strain PA11803

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: PA11803_RS25740
• Protein ID: WP_016263850.1
• Coordinates: 5402183..5402530 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: PA11803_RS25745
• Protein ID: WP_003114155.1
• Coordinates: 5402540..5402791 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA11803_RS25715	5397829..5398626	-	798	WP_033940878.1	IS21-like element helper ATPase IstB	-
PA11803_RS25720	5398616..5400142	-	1527	WP_033940880.1	IS21 family transposase	-
PA11803_RS25725	5400246..5400791	+	546	Protein_5145	LysR family substrate-binding domain-containing protein	-
PA11803_RS25730	5400827..5401132	-	306	WP_033940882.1	helix-turn-helix domain-containing protein	-
PA11803_RS25735	5401254..5401895	+	642	WP_033940883.1	substrate-binding domain-containing protein	-
PA11803_RS25740	5402183..5402530	-	348	WP_016263850.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA11803_RS25745	5402540..5402791	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PA11803_RS25750	5403005..5403988	-	984	WP_019725833.1	tyrosine-type recombinase/integrase	-
PA11803_RS25755	5403988..5405280	-	1293	WP_003115206.1	hypothetical protein	-
PA11803_RS25760	5405539..5406801	-	1263	WP_019725831.1	hypothetical protein	-
PA11803_RS25765	5406803..5407153	-	351	WP_019725830.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5310317..5414416	104099

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13046.85 Da
• Isoelectric Point: 4.4212

>T61997 WP_016263850.1 NZ_CP015003:c5402530-5402183 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T61997 NZ_CP015003:c5402530-5402183 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGTTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT61997 WP_003114155.1 NZ_CP015003:c5402791-5402540 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT61997 NZ_CP015003:c5402791-5402540 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACTTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355