Detailed information of TA system TA061990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 5203723..5204331
• Replicon: chromosome
• Accession: NZ_CP015002
• Organism: Pseudomonas aeruginosa strain PA8281

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: PA8281_RS24285
• Protein ID: WP_016263850.1
• Coordinates: 5203723..5204070 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: PA8281_RS24290
• Protein ID: WP_003114155.1
• Coordinates: 5204080..5204331 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA8281_RS24260	5199369..5200166	-	798	WP_033940878.1	IS21-like element helper ATPase IstB	-
PA8281_RS24265	5200156..5201682	-	1527	WP_033940880.1	IS21 family transposase	-
PA8281_RS24270	5201786..5202331	+	546	Protein_4843	LysR family substrate-binding domain-containing protein	-
PA8281_RS24275	5202367..5202672	-	306	WP_033940882.1	helix-turn-helix domain-containing protein	-
PA8281_RS24280	5202794..5203435	+	642	WP_033940883.1	substrate-binding domain-containing protein	-
PA8281_RS24285	5203723..5204070	-	348	WP_016263850.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA8281_RS24290	5204080..5204331	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PA8281_RS24295	5204545..5205528	-	984	WP_019725833.1	tyrosine-type recombinase/integrase	-
PA8281_RS24300	5205528..5206820	-	1293	WP_003115206.1	hypothetical protein	-
PA8281_RS24305	5207079..5208341	-	1263	WP_019725831.1	hypothetical protein	-
PA8281_RS24310	5208343..5208693	-	351	WP_019725830.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5111857..5215956	104099

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13046.85 Da
• Isoelectric Point: 4.4212

>T61990 WP_016263850.1 NZ_CP015002:c5204070-5203723 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T61990 NZ_CP015002:c5204070-5203723 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGTTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT61990 WP_003114155.1 NZ_CP015002:c5204331-5204080 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT61990 NZ_CP015002:c5204331-5204080 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACTTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355