Detailed information of TA system TA061982

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 5013969..5014577
• Replicon: chromosome
• Accession: NZ_CP015001
• Organism: Pseudomonas aeruginosa strain PA1088

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: PA1088_RS23255
• Protein ID: WP_016263850.1
• Coordinates: 5013969..5014316 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: PA1088_RS23260
• Protein ID: WP_003114155.1
• Coordinates: 5014326..5014577 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA1088_RS23225	5009328..5009600	+	273	WP_019725834.1	cysteine-rich CWC family protein	-
PA1088_RS23230	5009600..5010292	+	693	WP_003098362.1	16S rRNA pseudouridine(516) synthase	-
PA1088_RS23235	5010428..5011471	+	1044	WP_003098363.1	L,D-transpeptidase	-
PA1088_RS23240	5011551..5012288	+	738	WP_003114158.1	murein L,D-transpeptidase catalytic domain family protein	-
PA1088_RS23245	5012740..5013642	+	903	WP_003123042.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
PA1088_RS23255	5013969..5014316	-	348	WP_016263850.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA1088_RS23260	5014326..5014577	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PA1088_RS23265	5014791..5015774	-	984	WP_019725833.1	tyrosine-type recombinase/integrase	-
PA1088_RS23270	5015774..5017066	-	1293	WP_003115206.1	hypothetical protein	-
PA1088_RS23275	5017325..5018587	-	1263	WP_019725831.1	hypothetical protein	-
PA1088_RS23280	5018589..5018939	-	351	WP_019725830.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	5013969..5036112	22143

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13046.85 Da
• Isoelectric Point: 4.4212

>T61982 WP_016263850.1 NZ_CP015001:c5014316-5013969 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T61982 NZ_CP015001:c5014316-5013969 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGTTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT61982 WP_003114155.1 NZ_CP015001:c5014577-5014326 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT61982 NZ_CP015001:c5014577-5014326 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACTTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355