Detailed information of TA system TA061974

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 5266990..5267598
• Replicon: chromosome
• Accession: NZ_CP014999
• Organism: Pseudomonas aeruginosa strain PA7790

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: PA7790_RS24685
• Protein ID: WP_016263850.1
• Coordinates: 5266990..5267337 (-)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: PA7790_RS34170
• Protein ID: WP_003114155.1
• Coordinates: 5267347..5267598 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PA7790_RS24665	5262636..5263433	-	798	WP_033940878.1	IS21-like element helper ATPase IstB	-
PA7790_RS24670	5263423..5264949	-	1527	WP_033940880.1	IS21 family transposase	-
PA7790_RS24675	5265053..5265598	+	546	Protein_4929	LysR family substrate-binding domain-containing protein	-
PA7790_RS34165	5265634..5265939	-	306	WP_033940882.1	helix-turn-helix domain-containing protein	-
PA7790_RS24680	5266061..5266702	+	642	WP_033940883.1	substrate-binding domain-containing protein	-
PA7790_RS24685	5266990..5267337	-	348	WP_016263850.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PA7790_RS34170	5267347..5267598	-	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
PA7790_RS24690	5267812..5268795	-	984	WP_019725833.1	tyrosine-type recombinase/integrase	-
PA7790_RS24695	5268795..5270087	-	1293	WP_003115206.1	hypothetical protein	-
PA7790_RS24700	5270346..5271608	-	1263	WP_019725831.1	hypothetical protein	-
PA7790_RS24705	5271610..5271960	-	351	WP_019725830.1	DUF2523 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5134192..5279223	145031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13046.85 Da
• Isoelectric Point: 4.4212

>T61974 WP_016263850.1 NZ_CP014999:c5267337-5266990 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T61974 NZ_CP014999:c5267337-5266990 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGTTCCATTCCA
AGGTGAACAGGCTGCATTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT61974 WP_003114155.1 NZ_CP014999:c5267598-5267347 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT61974 NZ_CP014999:c5267598-5267347 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACTTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0B0C355