Detailed information of TA system TA061965

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1203903..1204820
• Replicon: chromosome
• Accession: NZ_CP014990
• Organism: Bacillus velezensis strain KD1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: A2I97_RS06310
• Protein ID: WP_057079950.1
• Coordinates: 1204074..1204820 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: -
• Locus tag: A2I97_RS06305
• Protein ID: WP_070081684.1
• Coordinates: 1203903..1204073 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2I97_RS06270	1199136..1200758	+	1623	WP_109955178.1	pyocin knob domain-containing protein	-
A2I97_RS06275	1200771..1201142	+	372	WP_070081681.1	hypothetical protein	-
A2I97_RS06280	1201147..1201344	+	198	WP_070081682.1	XkdX family protein	-
A2I97_RS06285	1201401..1202162	+	762	WP_070081683.1	phage portal protein	-
A2I97_RS06290	1202214..1202477	+	264	WP_003154815.1	hemolysin XhlA family protein	-
A2I97_RS06295	1202491..1202754	+	264	WP_003154813.1	phage holin	-
A2I97_RS06300	1202768..1203646	+	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
A2I97_RS06305	1203903..1204073	-	171	WP_070081684.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
A2I97_RS06310	1204074..1204820	-	747	WP_057079950.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
A2I97_RS06315	1204925..1205923	-	999	WP_003154805.1	inorganic phosphate transporter	-
A2I97_RS06320	1205936..1206553	-	618	WP_003154804.1	DUF47 domain-containing protein	-
A2I97_RS06325	1206839..1208155	-	1317	WP_003154801.1	amino acid permease	-
A2I97_RS06335	1208479..1209429	+	951	WP_069448685.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29033.52 Da
• Isoelectric Point: 4.7755

>T61965 WP_057079950.1 NZ_CP014990:c1204820-1204074 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T61965 NZ_CP014990:c1204820-1204074 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCAGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCAGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6704.79 Da
• Isoelectric Point: 10.9273

>AT61965 WP_070081684.1 NZ_CP014990:c1204073-1203903 [Bacillus velezensis]
MEQVFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT61965 NZ_CP014990:c1204073-1203903 [Bacillus velezensis]
ATGGAGCAGGTATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA