Detailed information of TA system TA061964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 477133..477770
• Replicon: chromosome
• Accession: NZ_CP014990
• Organism: Bacillus velezensis strain KD1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: A2I97_RS02485
• Protein ID: WP_003156187.1
• Coordinates: 477420..477770 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: A2I97_RS02480
• Protein ID: WP_003156188.1
• Coordinates: 477133..477414 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A2I97_RS02460	473498..474097	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
A2I97_RS02465	474190..474555	+	366	WP_070081400.1	holo-ACP synthase	-
A2I97_RS02470	474720..475727	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
A2I97_RS02475	475844..477013	+	1170	WP_070081401.1	alanine racemase	-
A2I97_RS02480	477133..477414	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
A2I97_RS02485	477420..477770	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
A2I97_RS02490	477888..478709	+	822	WP_003156182.1	STAS domain-containing protein	-
A2I97_RS02495	478714..479079	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
A2I97_RS02500	479082..479483	+	402	WP_003156178.1	anti-sigma regulatory factor	-
A2I97_RS02505	479495..480502	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
A2I97_RS02510	480566..480895	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
A2I97_RS02515	480892..481374	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
A2I97_RS02520	481340..482128	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
A2I97_RS02525	482128..482730	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T61964 WP_003156187.1 NZ_CP014990:477420-477770 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T61964 NZ_CP014990:477420-477770 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT61964 WP_003156188.1 NZ_CP014990:477133-477414 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT61964 NZ_CP014990:477133-477414 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5