Detailed information of TA system TA061957

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1776437..1776966
• Replicon: chromosome
• Accession: NZ_CP014989
• Organism: Serinicoccus hydrothermalis strain JLT9

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A1B1NCA9
• Locus tag: SGUI_RS08195
• Protein ID: WP_022923225.1
• Coordinates: 1776640..1776966 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: SGUI_RS08190
• Protein ID: WP_066638586.1
• Coordinates: 1776437..1776643 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SGUI_RS08155	1771536..1771889	-	354	WP_066638572.1	hypothetical protein	-
SGUI_RS17405	1772023..1772787	+	765	WP_157621777.1	hypothetical protein	-
SGUI_RS08170	1772940..1773605	-	666	WP_191090968.1	hypothetical protein	-
SGUI_RS08175	1773910..1774662	-	753	WP_157621779.1	hypothetical protein	-
SGUI_RS08185	1775505..1775921	-	417	WP_066638581.1	hypothetical protein	-
SGUI_RS08190	1776437..1776643	+	207	WP_066638586.1	DUF3018 family protein	Antitoxin
SGUI_RS08195	1776640..1776966	+	327	WP_022923225.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SGUI_RS08200	1777050..1777493	+	444	WP_083190576.1	NUDIX domain-containing protein	-
SGUI_RS08205	1777588..1778094	+	507	WP_202816615.1	GNAT family N-acetyltransferase	-
SGUI_RS08210	1778182..1779084	-	903	WP_066638590.1	NAD(P)-dependent oxidoreductase	-
SGUI_RS08215	1779139..1779729	+	591	WP_066638593.1	dihydrofolate reductase family protein	-
SGUI_RS08220	1779966..1780181	+	216	WP_066638594.1	DUF4177 domain-containing protein	-
SGUI_RS08225	1780151..1780579	-	429	WP_202816616.1	DUF4260 family protein	-
SGUI_RS08230	1780792..1781700	+	909	WP_066643044.1	arginase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1726116..1784036	57920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-106)

Antitoxin

• Antitoxin MazE-like (5-66)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11713.53 Da
• Isoelectric Point: 7.0141

>T61957 WP_022923225.1 NZ_CP014989:1776640-1776966 [Serinicoccus hydrothermalis]
VRRGELWTVAGGVYASKPRPALIVQDDLFEGTASVTVAPLTTTLVDAPLLRVRVAADDFTGLQHDSQVMIDKVTTVRREN
VGTRIGRLAPESLVEVERALMAFLGLAR

Nucleotide

• Length: 327 bp

>T61957 NZ_CP014989:1776640-1776966 [Serinicoccus hydrothermalis]
GTGAGGCGCGGCGAGCTGTGGACCGTCGCCGGGGGTGTCTACGCCTCCAAGCCGAGGCCCGCACTGATCGTGCAGGACGA
CCTCTTCGAGGGCACTGCCTCGGTGACCGTGGCGCCCCTGACGACGACGCTGGTAGATGCCCCTCTGCTTCGCGTACGCG
TCGCCGCCGATGATTTCACCGGCCTGCAGCACGACAGCCAGGTCATGATCGACAAGGTCACCACAGTCCGCCGTGAGAAC
GTCGGTACCCGCATTGGCCGCCTGGCGCCCGAAAGTCTCGTGGAGGTCGAACGCGCCCTCATGGCTTTCCTCGGCCTGGC
TAGGTAG

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 8055.96 Da
• Isoelectric Point: 5.1425

>AT61957 WP_066638586.1 NZ_CP014989:1776437-1776643 [Serinicoccus hydrothermalis]
MTVKHRVSEYRRRMRARGLRPVQIWVPDVRSERFAQEAERQAALVARADEQSDDQEFVEAVTAPWDEE

Nucleotide

• Length: 207 bp

>AT61957 NZ_CP014989:1776437-1776643 [Serinicoccus hydrothermalis]
ATGACGGTGAAGCATCGAGTCAGCGAGTACCGGCGCAGGATGCGCGCACGCGGACTTCGACCCGTGCAGATCTGGGTGCC
GGACGTGCGTAGCGAGCGCTTCGCACAGGAGGCCGAACGTCAGGCGGCCCTGGTCGCCAGGGCGGACGAGCAGTCCGATG
ACCAGGAGTTCGTCGAAGCCGTCACCGCCCCCTGGGACGAGGAGTGA

Structures

Toxin

• AlphaFold DB: A0A1B1NCA9