61945

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 2307748..2308330 Replicon chromosome
Accession NZ_CP014949
Organism Enterococcus faecalis strain LD33

Toxin (Protein)


Gene name zeta Uniprot ID A0A0M2AE74
Locus tag A3777_RS11470 Protein ID WP_002355414.1
Coordinates 2308022..2308330 (+) Length 103 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID Q9AL19
Locus tag A3777_RS11465 Protein ID WP_002326825.1
Coordinates 2307748..2308020 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3777_RS11440 2303029..2303757 - 729 WP_002355404.1 GntR family transcriptional regulator -
A3777_RS11445 2303934..2304860 + 927 WP_002355406.1 hypothetical protein -
A3777_RS11450 2304877..2306160 + 1284 WP_002363033.1 PTS sugar transporter subunit IIC -
A3777_RS14375 2306352..2306474 + 123 Protein_2160 topoisomerase -
A3777_RS11455 2306549..2307445 + 897 WP_002363034.1 ParA family protein -
A3777_RS11460 2307522..2307731 + 210 WP_002363035.1 peptide-binding protein -
A3777_RS11465 2307748..2308020 + 273 WP_002326825.1 antitoxin Antitoxin
A3777_RS11470 2308022..2308330 + 309 WP_002355414.1 zeta toxin family protein Toxin
A3777_RS14380 2308410..2308832 - 423 WP_080005963.1 tyrosine-type recombinase/integrase -
A3777_RS11475 2308884..2309384 - 501 WP_002363039.1 HAD family hydrolase -
A3777_RS11480 2309389..2310156 - 768 WP_002355416.1 DeoR/GlpR family DNA-binding transcription regulator -
A3777_RS11490 2310643..2311068 + 426 WP_002355418.1 galactose-6-phosphate isomerase subunit LacA -
A3777_RS11495 2311085..2311600 + 516 WP_002345825.1 galactose-6-phosphate isomerase subunit LacB -
A3777_RS11500 2311611..2312543 + 933 WP_002363040.1 tagatose-6-phosphate kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element ClpL bsh / esp 2275561..2366212 90651


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-99)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11525.91 Da        Isoelectric Point: 5.7251

>T61945 WP_002355414.1 NZ_CP014949:2308022-2308330 [Enterococcus faecalis]
MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGSGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELV
KLYEKDVVKHATSYSNQLVKLN

Download         Length: 309 bp

>T61945 NZ_CP014949:2308022-2308330 [Enterococcus faecalis]
ATGGCGAATATTACTGACTTCACCGAAAAGCAATTTGAAGATCGTTTAGAAAAGAATGTTGAACGACTAACTAAAAATAG
ACTAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGACAACCAGGATCAGGGAAAACTAGTTTGCGATCGGCAATTT
CCGAAGAAACACAAGGAAATGTTGTTATCATTGATAATGATACGTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACATCCTATTCTAATCAGCTGGTAAAGCTTAATTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10720.17 Da        Isoelectric Point: 4.4865

>AT61945 WP_002326825.1 NZ_CP014949:2307748-2308020 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEVNLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYS
KQILTKEKVA

Download         Length: 273 bp

>AT61945 NZ_CP014949:2307748-2308020 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACATTTGAAATTGAAATCATTAATGAGTTATCGGCAAGTGTTTATAATCGGGTATTAAA
TTATGTTTTGAATCATGAACTAGATACTAAAAATACTCGTTTACTAGAAGTGAATCTTTTAAATCAATTAGAAGTGGCAC
AAGAAGTTGATTTATTTCAACAACCATTTGAAGAATTACAAGCTATTCATGAGTATTGGCGGTCAATGAATCAATATTCA
AAACAAATTTTGACAAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AE74

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AF93

References