Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1799021..1799592 Replicon chromosome
Accession NZ_CP014949
Organism Enterococcus faecalis strain LD33

Toxin (Protein)


Gene name mazF Uniprot ID R3GRA7
Locus tag A3777_RS08865 Protein ID WP_002360937.1
Coordinates 1799021..1799362 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S4CGQ1
Locus tag A3777_RS08870 Protein ID WP_002367500.1
Coordinates 1799362..1799592 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3777_RS08860 1795036..1798650 - 3615 WP_002389470.1 DNA-directed RNA polymerase subunit beta -
A3777_RS08865 1799021..1799362 - 342 WP_002360937.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
A3777_RS08870 1799362..1799592 - 231 WP_002367500.1 hypothetical protein Antitoxin
A3777_RS14570 1799647..1799826 - 180 WP_111997057.1 hypothetical protein -
A3777_RS08880 1800006..1800221 - 216 WP_002354768.1 zinc ribbon domain-containing protein -
A3777_RS08885 1800360..1801352 + 993 WP_002389493.1 biotin--[acetyl-CoA-carboxylase] ligase -
A3777_RS08890 1801616..1802254 - 639 WP_002378863.1 lytic polysaccharide monooxygenase -
A3777_RS08900 1802940..1804556 + 1617 WP_002389442.1 phosphatase PAP2/LCP family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13165.41 Da        Isoelectric Point: 9.3988

>T61927 WP_002360937.1 NZ_CP014949:c1799362-1799021 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T61927 NZ_CP014949:c1799362-1799021 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8704.69 Da        Isoelectric Point: 4.3313

>AT61927 WP_002367500.1 NZ_CP014949:c1799592-1799362 [Enterococcus faecalis]
MLTTKSRRQGNSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Download         Length: 231 bp

>AT61927 NZ_CP014949:c1799592-1799362 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2A7G9

Antitoxin

Source ID Structure
AlphaFold DB S4CGQ1

References