Detailed information of TA system TA061919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/Peptidase_M78-HTH_26
• Location: 1437386..1438084
• Replicon: chromosome
• Accession: NZ_CP014949
• Organism: Enterococcus faecalis strain LD33

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: A0A828QMF1
• Locus tag: A3777_RS07175
• Protein ID: WP_002388207.1
• Coordinates: 1437740..1438084 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: A3777_RS07170
• Protein ID: WP_002388206.1
• Coordinates: 1437386..1437721 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3777_RS07110	1432897..1433145	-	249	WP_002357361.1	hypothetical protein	-
A3777_RS07120	1433280..1433798	-	519	WP_062775408.1	DUF1642 domain-containing protein	-
A3777_RS07125	1433812..1434090	-	279	WP_062775410.1	hypothetical protein	-
A3777_RS14655	1434087..1434248	-	162	WP_195912343.1	hypothetical protein	-
A3777_RS07130	1434248..1434583	-	336	WP_062775412.1	hypothetical protein	-
A3777_RS07135	1434584..1434997	-	414	WP_062775509.1	hypothetical protein	-
A3777_RS07140	1434981..1435367	-	387	Protein_1361	hypothetical protein	-
A3777_RS07145	1435360..1436046	-	687	WP_062775415.1	Rha family transcriptional regulator	-
A3777_RS07150	1436128..1436397	-	270	WP_002365131.1	hypothetical protein	-
A3777_RS07155	1436473..1436787	+	315	WP_010776102.1	hypothetical protein	-
A3777_RS07165	1436912..1437094	-	183	WP_002388205.1	hypothetical protein	-
A3777_RS07170	1437386..1437721	+	336	WP_002388206.1	helix-turn-helix domain-containing protein	Antitoxin
A3777_RS07175	1437740..1438084	+	345	WP_002388207.1	ImmA/IrrE family metallo-endopeptidase	Toxin
A3777_RS07180	1438109..1438342	-	234	WP_002367290.1	hypothetical protein	-
A3777_RS07185	1438432..1439115	+	684	WP_129194668.1	hypothetical protein	-
A3777_RS07190	1439291..1440472	+	1182	WP_062775417.1	site-specific integrase	-
A3777_RS07195	1440576..1440725	-	150	WP_002356321.1	50S ribosomal protein L33	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1419201..1440472	21271

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (21-86)

Antitoxin

• Cro/C1-type helix-turn-helix domain (5-60)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13625.58 Da
• Isoelectric Point: 4.9570

>T61919 WP_002388207.1 NZ_CP014949:1437740-1438084 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRACFDPTYGIIFVNQNLTPTEQEEAIYHELKHVEDHVDIMALYKIPVFRSKMESEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK

Nucleotide

• Length: 345 bp

>T61919 NZ_CP014949:1437740-1438084 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTAGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTGAACAAACGCGCATGTTTCGA
CCCTACTTACGGTATCATATTTGTAAATCAAAATTTAACACCAACAGAACAAGAAGAAGCAATATATCACGAATTAAAGC
ATGTAGAAGACCATGTGGATATAATGGCATTGTATAAAATTCCTGTTTTTCGTTCTAAGATGGAATCCGAAGCAGAACAA
TATATGTTTAGAAGCTTAATCGAAAAATATGAAGGACAATACAATTACTCAAATGTTATAGCTCATTACAACTTAAAAAT
GGGACAAGAAATTTATTTGAAATAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12842.51 Da
• Isoelectric Point: 5.2926

>AT61919 WP_002388206.1 NZ_CP014949:1437386-1437721 [Enterococcus faecalis]
MTVFDRIKLLAKERGKSLNRIEEELGLPKNVLYRMKTSDNPTKDRLETLANYFDVSVDYLLGRTDNTKATDEKKSDDLDD
VLDNVMSFDGKPLDDHDREVIRAYLKGRFGK

Nucleotide

• Length: 336 bp

>AT61919 NZ_CP014949:1437386-1437721 [Enterococcus faecalis]
ATGACAGTGTTTGATAGGATCAAATTATTAGCAAAAGAGAGAGGAAAATCCTTAAATCGAATCGAAGAAGAATTAGGTTT
ACCAAAAAATGTTCTGTATCGTATGAAAACTTCCGATAATCCTACTAAAGATAGACTAGAAACTTTAGCTAATTACTTTG
ATGTATCTGTAGATTATCTATTGGGAAGAACTGATAATACTAAAGCAACTGATGAAAAAAAATCTGATGATTTAGATGAT
GTACTGGATAACGTCATGAGCTTTGACGGTAAACCGCTTGATGATCATGACAGAGAAGTTATCCGTGCATATTTAAAGGG
TAGATTCGGGAAATAA

Structures

Toxin

• AlphaFold DB: A0A828QMF1