Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09812-MazE
Location 1112032..1112663 Replicon chromosome
Accession NZ_CP014947
Organism Pseudomonas koreensis strain D26

Toxin (Protein)


Gene name mazF Uniprot ID A0A1N7CQ04
Locus tag AYO71_RS04970 Protein ID WP_041477600.1
Coordinates 1112301..1112663 (+) Length 121 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID J2YH56
Locus tag AYO71_RS04965 Protein ID WP_008077592.1
Coordinates 1112032..1112301 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYO71_RS04930 1107531..1108076 - 546 WP_041477595.1 membrane protein -
AYO71_RS04935 1108073..1108327 - 255 WP_041477596.1 acyl carrier protein -
AYO71_RS04940 1108337..1108612 - 276 WP_039756723.1 acyl carrier protein -
AYO71_RS04945 1108590..1109402 - 813 WP_041477597.1 1-acyl-sn-glycerol-3-phosphate acyltransferase -
AYO71_RS04950 1109378..1110103 - 726 WP_041477598.1 beta-ketoacyl synthase chain length factor -
AYO71_RS04955 1110460..1111230 + 771 WP_024011296.1 ParA family protein -
AYO71_RS04960 1111361..1111795 - 435 WP_041477599.1 thioredoxin TrxC -
AYO71_RS04965 1112032..1112301 + 270 WP_008077592.1 hypothetical protein Antitoxin
AYO71_RS04970 1112301..1112663 + 363 WP_041477600.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AYO71_RS04975 1112669..1114132 - 1464 WP_041477601.1 YdiU family protein -
AYO71_RS04980 1114181..1117534 - 3354 WP_041477602.1 mechanosensitive channel MscK -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-115)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 121 a.a.        Molecular weight: 13337.24 Da        Isoelectric Point: 7.1674

>T61903 WP_041477600.1 NZ_CP014947:1112301-1112663 [Pseudomonas koreensis]
MVRRQPPQRGDVYWIDPNPVAGREMMNRHRFVVITPREINALGVSMTVPVTSGGNFSRNMGLAVAITGHETNGVAVCNQV
RSFDIEQRVRDGTAKFIERLDDVTMSDIVDRVISAIDPLS

Download         Length: 363 bp

>T61903 NZ_CP014947:1112301-1112663 [Pseudomonas koreensis]
ATGGTGCGCCGACAGCCCCCTCAGCGGGGTGACGTTTACTGGATTGATCCAAACCCAGTGGCGGGGCGGGAGATGATGAA
CAGACATCGCTTTGTGGTGATCACGCCCAGAGAGATCAACGCATTGGGCGTGAGCATGACGGTGCCTGTTACTAGCGGTG
GGAATTTCAGCCGGAATATGGGTTTGGCGGTCGCCATTACTGGCCACGAAACCAATGGTGTAGCGGTGTGTAATCAGGTT
CGCAGTTTTGACATAGAACAACGGGTACGAGACGGCACGGCCAAATTCATCGAGCGACTGGATGACGTGACAATGTCTGA
CATCGTCGACCGTGTAATCAGTGCAATCGACCCGCTAAGCTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9521.94 Da        Isoelectric Point: 5.1069

>AT61903 WP_008077592.1 NZ_CP014947:1112032-1112301 [Pseudomonas koreensis]
MSVTSKIRRQGGAAVITIPPTLLKLLHLEVGAQLELNVVDGELIARPAVAATRKRYSLSELLEGAEELQKLNAQTSETLE
GDSVGRELG

Download         Length: 270 bp

>AT61903 NZ_CP014947:1112032-1112301 [Pseudomonas koreensis]
ATGTCCGTAACTTCCAAGATACGCCGCCAAGGCGGCGCTGCCGTCATTACGATTCCGCCCACGCTTTTAAAGTTGTTGCA
TCTGGAGGTTGGCGCACAGCTAGAGCTGAATGTGGTCGATGGCGAACTGATTGCTCGTCCAGCCGTAGCCGCTACGCGTA
AGCGTTATAGTTTGTCAGAGCTACTCGAAGGCGCAGAAGAATTGCAGAAACTTAACGCCCAGACTAGCGAGACCCTTGAA
GGTGATTCTGTCGGCCGGGAGCTGGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1N7CQ04

Antitoxin

Source ID Structure
AlphaFold DB J2YH56

References