Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 96958..98582 Replicon chromosome
Accession NZ_CP014947
Organism Pseudomonas koreensis strain D26

Toxin (Protein)


Gene name hipA Uniprot ID A0A554AI75
Locus tag AYO71_RS00405 Protein ID WP_041476581.1
Coordinates 96958..98307 (+) Length 450 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A554AI87
Locus tag AYO71_RS00410 Protein ID WP_041476582.1
Coordinates 98304..98582 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYO71_RS00395 92349..94157 + 1809 WP_041476579.1 sodium-extruding oxaloacetate decarboxylase subunit alpha -
AYO71_RS00400 94369..96588 - 2220 WP_041476580.1 autotransporter outer membrane beta-barrel domain-containing protein -
AYO71_RS29475 96491..96715 - 225 WP_167351289.1 hypothetical protein -
AYO71_RS00405 96958..98307 + 1350 WP_041476581.1 HipA domain-containing protein Toxin
AYO71_RS00410 98304..98582 + 279 WP_041476582.1 helix-turn-helix domain-containing protein Antitoxin
AYO71_RS00415 98590..99426 - 837 WP_041476583.1 amino acid ABC transporter permease -
AYO71_RS00420 99423..100241 - 819 WP_041476584.1 ABC transporter substrate-binding protein -
AYO71_RS00425 100355..101254 + 900 WP_041476585.1 homocysteine S-methyltransferase family protein -
AYO71_RS00430 101350..102021 - 672 WP_041476586.1 GNAT family N-acetyltransferase -
AYO71_RS00435 102034..102480 - 447 WP_041476587.1 GNAT family N-acetyltransferase -
AYO71_RS00440 102491..103114 - 624 WP_041476659.1 FMN-binding negative transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(165-367)

Antitoxin

Cro/C1-type helix-turn-helix domain

(27-80)


Sequences

Toxin        


Download         Length: 450 a.a.        Molecular weight: 49892.57 Da        Isoelectric Point: 5.1645

>T61901 WP_041476581.1 NZ_CP014947:96958-98307 [Pseudomonas koreensis]
MYKLTLQIFTAGQWHDAMILDFDDPEKGFESRCSFGYEADYLVENIELIGSPFSQAVSALYPLDWEGRRAKTPAFVHDIA
PAGAAKRFLLARLGQERPAEISADLFLLGRSTPAPIGNMRIKESAEAVDERKPIGFKREEIISRDNRFLEYAYELGAAIG
GATGAGGEAPKLLLAENSAGLLYPDAVLDDEEVRQHWFVKFARNRGGQTDQDILRSEFHYYKALQTLGIETVAAEGLALE
EATKPSLWMRRFDRQVKDGAVSRFAVESIYSLAEVTTPGSAMNHLEVIRMLAGLWRKAGQASQIPGLVADYLRRDLINKI
LGNSDNHGRNTAIIRQENAFQLAPIYDLAPMVMDDEGVTRTTKWPKELERAGDVDWRGVCRVLADSADPHESLTGLDASA
LFEGLRADARRLAALPDILAASGLPARTMNHPSVHLKNLEQRLKEWDLQ

Download         Length: 1350 bp

>T61901 NZ_CP014947:96958-98307 [Pseudomonas koreensis]
ATGTATAAGCTGACACTCCAGATCTTCACCGCCGGGCAATGGCACGACGCGATGATTCTTGACTTCGATGATCCGGAAAA
AGGCTTCGAAAGCCGCTGCAGTTTTGGCTATGAAGCGGACTATCTGGTGGAAAACATCGAGTTGATCGGTTCGCCTTTTT
CCCAAGCCGTGAGTGCCCTGTATCCACTGGATTGGGAAGGTCGGCGCGCGAAGACGCCGGCCTTTGTCCACGACATCGCA
CCGGCAGGCGCAGCCAAGCGGTTTCTGCTCGCCCGCCTGGGCCAGGAAAGACCCGCCGAGATCAGCGCCGATCTGTTTCT
GCTGGGTCGCAGTACACCGGCACCCATTGGCAACATGCGAATAAAGGAATCGGCGGAAGCAGTCGATGAGAGGAAGCCGA
TCGGATTCAAGCGCGAGGAGATCATTAGCCGCGACAACCGCTTTCTTGAATATGCCTACGAGCTGGGCGCGGCCATCGGT
GGCGCGACGGGGGCAGGTGGGGAAGCTCCGAAGCTCTTACTGGCAGAGAACAGCGCCGGGCTTCTGTATCCCGATGCGGT
ATTGGACGATGAAGAAGTGCGACAGCACTGGTTCGTGAAATTCGCCAGAAACAGGGGCGGGCAGACCGACCAGGACATCC
TGCGCAGCGAATTCCATTACTACAAAGCGCTGCAGACATTGGGCATCGAAACGGTCGCGGCAGAAGGCCTGGCCTTGGAA
GAGGCGACCAAACCGAGTTTGTGGATGCGGCGTTTTGATCGACAAGTGAAAGATGGGGCGGTGTCGCGTTTCGCCGTCGA
ATCGATTTATTCGCTGGCTGAAGTGACGACGCCAGGCAGCGCCATGAATCATCTGGAAGTGATCCGCATGCTCGCCGGGC
TCTGGCGCAAAGCGGGTCAGGCCAGCCAGATTCCCGGCCTCGTCGCCGATTATCTGCGTCGCGACCTGATCAATAAAATC
CTCGGAAACTCCGATAACCACGGTCGTAACACGGCGATCATCCGGCAAGAGAACGCTTTCCAGCTCGCGCCGATTTATGA
CCTCGCCCCGATGGTCATGGATGACGAAGGCGTGACGCGTACGACCAAATGGCCAAAAGAGTTGGAGCGGGCGGGAGATG
TCGATTGGCGAGGTGTCTGCCGCGTATTGGCCGATAGCGCAGATCCGCACGAATCTTTGACGGGGCTGGATGCCTCGGCG
CTGTTCGAAGGCTTGCGCGCGGATGCCCGCCGTCTGGCCGCACTGCCGGATATTCTTGCGGCCAGCGGTTTGCCCGCCAG
AACCATGAATCACCCGAGCGTTCATCTGAAAAACCTCGAGCAACGTTTGAAAGAGTGGGACCTGCAATGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10332.12 Da        Isoelectric Point: 10.7364

>AT61901 WP_041476582.1 NZ_CP014947:98304-98582 [Pseudomonas koreensis]
MSLTVLERTALIESIQQSLAEGTLEIGDAVRRLRVEVTGLHQTQFAKMCKISVRTLVHIEHGEGNQTLKSLNAVFRPFGM
KMGVVRIRRDTI

Download         Length: 279 bp

>AT61901 NZ_CP014947:98304-98582 [Pseudomonas koreensis]
ATGAGTCTGACCGTCCTCGAGCGCACGGCGCTCATCGAAAGCATTCAGCAATCACTGGCCGAAGGCACTCTGGAAATCGG
TGACGCCGTGCGCCGTTTACGGGTCGAAGTCACCGGTCTGCATCAGACCCAGTTCGCGAAGATGTGCAAGATTTCAGTGC
GCACGCTGGTGCATATCGAACATGGCGAAGGCAATCAGACCCTGAAGTCGCTGAACGCGGTGTTCCGCCCCTTCGGCATG
AAAATGGGCGTAGTGCGGATTCGCCGGGACACCATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A554AI75

Antitoxin

Source ID Structure
AlphaFold DB A0A554AI87

References