Detailed information of TA system TA061896

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK(toxin)
• Location: 422061..422626
• Replicon: plasmid unnamed
• Accession: NZ_CP014942
• Organism: Rhodococcus sp. BH4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: A0W34_RS31310
• Protein ID: WP_076948937.1
• Coordinates: 422270..422626 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: A0W34_RS31305
• Protein ID: WP_076948938.1
• Coordinates: 422061..422273 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0W34_RS31275	417301..417879	+	579	WP_064688400.1	CBS domain-containing protein	-
A0W34_RS31280	417928..419298	+	1371	WP_081559340.1	DUF1298 domain-containing protein	-
A0W34_RS31285	419292..420089	+	798	WP_081559342.1	MFS transporter	-
A0W34_RS32950	419998..420561	-	564	WP_198035744.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
A0W34_RS31295	421409..421657	+	249	WP_076948940.1	hypothetical protein	-
A0W34_RS31305	422061..422273	+	213	WP_076948938.1	ribbon-helix-helix domain-containing protein	Antitoxin
A0W34_RS31310	422270..422626	+	357	WP_076948937.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A0W34_RS31320	424231..426015	-	1785	WP_081559348.1	Hsp70 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..704258	704258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-114)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-39)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12788.57 Da
• Isoelectric Point: 7.4061

>T61896 WP_076948937.1 NZ_CP014942:422270-422626 [Rhodococcus sp. BH4]
MIRRAHIHWVDLDTAQGSEAAKRRPAVVVSNDRANATAENLGRGVITVVPLTANTAHVFPFQVMVEPDESGLARASKAQA
EQVRSVSVRRLDPAPVGKVGMETFAALEDALRLHLDLQ

Nucleotide

• Length: 357 bp

>T61896 NZ_CP014942:422270-422626 [Rhodococcus sp. BH4]
ATGATTCGGCGCGCACACATCCACTGGGTGGATCTCGATACCGCGCAGGGCAGTGAAGCGGCGAAACGACGACCGGCGGT
GGTCGTGAGCAACGACCGGGCCAACGCCACTGCGGAAAATCTCGGCAGGGGCGTGATCACCGTGGTTCCGTTGACTGCCA
ATACTGCGCACGTATTTCCGTTTCAGGTGATGGTCGAGCCCGACGAATCAGGTTTGGCGCGAGCGTCGAAAGCACAAGCG
GAGCAAGTGCGTTCGGTATCGGTTCGGCGTCTGGATCCTGCACCTGTCGGCAAGGTCGGGATGGAGACTTTCGCCGCACT
CGAGGACGCGCTGCGGCTGCACCTCGATCTGCAGTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 7821.63 Da
• Isoelectric Point: 4.2208

>AT61896 WP_076948938.1 NZ_CP014942:422061-422273 [Rhodococcus sp. BH4]
MTRKFTISLPDEDVHLLDEYVRDHGLRGRSAGIRAALRLLSTSGLERDYAIAFAEDDVDEVWDAVSADAE

Nucleotide

• Length: 213 bp

>AT61896 NZ_CP014942:422061-422273 [Rhodococcus sp. BH4]
ATGACCAGGAAATTTACCATAAGTCTTCCTGATGAGGATGTGCATCTACTCGACGAGTATGTGCGTGATCACGGGCTTCG
TGGTCGGTCGGCAGGAATTCGAGCTGCGCTCCGCCTTCTCTCGACGTCCGGACTCGAACGTGACTACGCCATCGCCTTTG
CTGAAGATGACGTAGATGAAGTGTGGGACGCTGTCTCGGCAGACGCCGAATGA