Detailed information of TA system TA061895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 334547..335082
• Replicon: plasmid unnamed
• Accession: NZ_CP014942
• Organism: Rhodococcus sp. BH4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A7I8BDH8
• Locus tag: A0W34_RS30900
• Protein ID: WP_042445814.1
• Coordinates: 334547..334846 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: A0W34_RS30905
• Protein ID: WP_064688354.1
• Coordinates: 334846..335082 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0W34_RS30885	330670..331740	+	1071	WP_081559267.1	DUF1016 family protein	-
A0W34_RS30890	331871..332668	-	798	WP_081559269.1	hypothetical protein	-
A0W34_RS30895	332668..334518	-	1851	WP_081559270.1	hypothetical protein	-
A0W34_RS30900	334547..334846	-	300	WP_042445814.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
A0W34_RS30905	334846..335082	-	237	WP_064688354.1	hypothetical protein	Antitoxin
A0W34_RS30910	335458..336132	-	675	WP_081559272.1	TetR/AcrR family transcriptional regulator	-
A0W34_RS30915	336188..337162	-	975	WP_081559273.1	DUF2637 domain-containing protein	-
A0W34_RS30920	337794..338108	-	315	WP_081559275.1	hypothetical protein	-
A0W34_RS30925	338105..338887	-	783	WP_029256883.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..704258	704258

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-92)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10441.94 Da
• Isoelectric Point: 4.3831

>T61895 WP_042445814.1 NZ_CP014942:c334846-334547 [Rhodococcus sp. BH4]
MRPIHEADLDTVRPVLVLTREVVRPHLGTVTVAPITDEIRGLSTEVSVSAANGLSADAVVSCDHITTIPVSALGNVIGYL
LPAQEESLSIAVRAAFDLE

Nucleotide

• Length: 300 bp

>T61895 NZ_CP014942:c334846-334547 [Rhodococcus sp. BH4]
ATGCGACCAATTCACGAAGCCGACCTCGACACAGTCCGCCCGGTCCTCGTACTCACCCGAGAGGTGGTCCGTCCACACCT
CGGGACCGTCACGGTAGCGCCCATCACCGACGAGATCCGAGGACTGTCCACTGAGGTGTCCGTCAGTGCCGCGAACGGAC
TATCCGCCGACGCGGTCGTCAGTTGCGATCACATCACCACCATTCCCGTCAGTGCACTCGGCAACGTCATCGGTTATCTT
CTGCCAGCACAAGAAGAATCACTGTCCATAGCCGTGCGCGCCGCATTCGATCTCGAATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8642.63 Da
• Isoelectric Point: 6.4809

>AT61895 WP_064688354.1 NZ_CP014942:c335082-334846 [Rhodococcus sp. BH4]
MSTQIAVRLPDEIVAFLDDEIREHRATSRASVVLRALERERRRQIAARDAEILANATSEHDLSSLAHHAANPQSTGLS

Nucleotide

• Length: 237 bp

>AT61895 NZ_CP014942:c335082-334846 [Rhodococcus sp. BH4]
ATGAGCACGCAGATCGCGGTACGACTACCGGACGAGATCGTGGCATTTCTCGACGACGAAATCAGGGAACATCGCGCCAC
CTCCCGGGCCTCGGTAGTGTTGCGCGCTCTCGAACGCGAACGTCGCCGTCAGATTGCCGCACGTGACGCGGAGATTCTCG
CGAACGCCACATCCGAACATGACCTGAGTTCTCTCGCTCACCACGCCGCCAATCCGCAGTCAACCGGCTTGAGCTGA

Structures

Toxin

• AlphaFold DB: A0A7I8BDH8