Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 13878..14479 Replicon plasmid pL11995-7
Accession NZ_CP014931
Organism Secundilactobacillus paracollinoides strain TMW 1.1995

Toxin (Protein)


Gene name pemK Uniprot ID A0A1B2J378
Locus tag AYR63_RS16490 Protein ID WP_065937815.1
Coordinates 13878..14222 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A806J926
Locus tag AYR63_RS16495 Protein ID WP_001748109.1
Coordinates 14216..14479 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR63_RS16460 8978..9997 + 1020 WP_065937810.1 hypothetical protein -
AYR63_RS16465 9987..11408 + 1422 WP_065937811.1 hypothetical protein -
AYR63_RS16470 11691..12035 + 345 WP_065937812.1 hypothetical protein -
AYR63_RS16475 12077..12625 + 549 WP_065937813.1 helix-turn-helix transcriptional regulator -
AYR63_RS16480 12625..13038 + 414 WP_065937814.1 protein-export chaperone SecB -
AYR63_RS16485 13094..13375 + 282 WP_062904674.1 DUF3892 domain-containing protein -
AYR63_RS17245 13496..13786 - 291 WP_083215625.1 type II toxin-antitoxin system PemK/MazF family toxin -
AYR63_RS16490 13878..14222 - 345 WP_065937815.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AYR63_RS16495 14216..14479 - 264 WP_001748109.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AYR63_RS16505 14726..15082 - 357 WP_002834562.1 type II toxin-antitoxin system YafQ family toxin -
AYR63_RS16510 15082..15360 - 279 WP_002834561.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
AYR63_RS16515 15437..16024 + 588 WP_065937816.1 site-specific integrase -
AYR63_RS16520 16076..16446 + 371 Protein_22 type II toxin-antitoxin system RelE/ParE family toxin -
AYR63_RS16525 16433..16702 + 270 WP_057911382.1 helix-turn-helix domain-containing protein -
AYR63_RS16530 16824..17522 + 699 WP_065937817.1 MobC family plasmid mobilization relaxosome protein -
AYR63_RS16535 17504..18673 + 1170 WP_065937818.1 relaxase/mobilization nuclease domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..19529 19529


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-101)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13088.82 Da        Isoelectric Point: 5.1121

>T61893 WP_065937815.1 NZ_CP014931:c14222-13878 [Secundilactobacillus paracollinoides]
MVSQGDIFYVNFNPSRGHEQMNRRPAIALSNDLVCQTSNMTIVAPISSTERNFPMYHRLNSSQTIHGKVLLDQTIALDLR
ARHVTDEAIVDHVSREELEEIIFLYKLLFSIDDE

Download         Length: 345 bp

>T61893 NZ_CP014931:c14222-13878 [Secundilactobacillus paracollinoides]
ATGGTAAGTCAGGGCGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAGGCGGCCGGCCAT
TGCTCTAAGTAATGATCTAGTCTGTCAAACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCGAGCGTAATTTTC
CTATGTATCATCGGCTAAATAGTAGTCAAACAATACATGGGAAAGTATTATTAGATCAAACAATAGCCTTAGATTTGCGG
GCAAGACATGTCACTGATGAAGCCATTGTTGATCATGTATCGCGTGAAGAATTAGAAGAAATAATCTTTTTATACAAATT
GTTGTTCAGTATTGATGACGAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.47 Da        Isoelectric Point: 5.7117

>AT61893 WP_001748109.1 NZ_CP014931:c14479-14216 [Secundilactobacillus paracollinoides]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT61893 NZ_CP014931:c14479-14216 [Secundilactobacillus paracollinoides]
ATGGAACTAATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAGATTATTAA
ACAATTGAAATTAGATGATAACCAAGATATGACAATAACAATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGACTGGCAAGATGACGGAAAACGTGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B2J378

Antitoxin

Source ID Structure
AlphaFold DB A0A806J926

References