Detailed information of TA system TA061892

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 5230..5831
• Replicon: plasmid pL11995-1
• Accession: NZ_CP014925
• Organism: Secundilactobacillus paracollinoides strain TMW 1.1995

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q684P1
• Locus tag: AYR63_RS15220
• Protein ID: WP_001748110.1
• Coordinates: 5487..5831 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A806J926
• Locus tag: AYR63_RS15215
• Protein ID: WP_001748109.1
• Coordinates: 5230..5493 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR63_RS15160	403..552	+	150	WP_156770510.1	hypothetical protein	-
AYR63_RS15165	545..907	+	363	WP_065904102.1	hypothetical protein	-
AYR63_RS15170	904..1098	+	195	WP_065904100.1	hypothetical protein	-
AYR63_RS15175	1098..1538	+	441	WP_065904097.1	hypothetical protein	-
AYR63_RS15180	1519..1710	+	192	WP_065904095.1	hypothetical protein	-
AYR63_RS15185	1754..2182	+	429	WP_065904091.1	transcriptional regulator	-
AYR63_RS15195	2663..3184	+	522	WP_065904088.1	HNH endonuclease	-
AYR63_RS15200	3377..3833	+	457	Protein_8	phage terminase small subunit P27 family	-
AYR63_RS15205	4179..4544	-	366	WP_065904087.1	hypothetical protein	-
AYR63_RS15210	4557..5144	-	588	WP_065904085.1	site-specific integrase	-
AYR63_RS15215	5230..5493	+	264	WP_001748109.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AYR63_RS15220	5487..5831	+	345	WP_001748110.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYR63_RS15225	5942..7189	-	1248	WP_065937709.1	restriction endonuclease subunit S	-
AYR63_RS15230	7174..7731	-	558	WP_065904083.1	HTH domain-containing protein	-
AYR63_RS15235	7724..8653	-	930	WP_065904080.1	replication initiation protein	-
AYR63_RS15240	9587..10756	-	1170	WP_065904053.1	relaxase/mobilization nuclease domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38125	38125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13041.89 Da
• Isoelectric Point: 6.4782

>T61892 WP_001748110.1 NZ_CP014925:5487-5831 [Secundilactobacillus paracollinoides]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T61892 NZ_CP014925:5487-5831 [Secundilactobacillus paracollinoides]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTGGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10141.47 Da
• Isoelectric Point: 5.7117

>AT61892 WP_001748109.1 NZ_CP014925:5230-5493 [Secundilactobacillus paracollinoides]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT61892 NZ_CP014925:5230-5493 [Secundilactobacillus paracollinoides]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAGCTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0R2K1X3

Antitoxin

• AlphaFold DB: A0A806J926