Detailed information of TA system TA061888

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 39327..39928
• Replicon: plasmid pL11979-1
• Accession: NZ_CP014913
• Organism: Secundilactobacillus paracollinoides strain TMW 1.1979

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: Q684P1
• Locus tag: AYR61_RS14495
• Protein ID: WP_001748110.1
• Coordinates: 39327..39671 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A806J926
• Locus tag: AYR61_RS14500
• Protein ID: WP_001748109.1
• Coordinates: 39665..39928 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR61_RS14475	34402..35571	+	1170	WP_065904053.1	relaxase/mobilization nuclease domain-containing protein	-
AYR61_RS14480	36504..37433	+	930	WP_065904080.1	replication initiation protein	-
AYR61_RS14485	37426..37983	+	558	WP_065904083.1	HTH domain-containing protein	-
AYR61_RS14490	37968..39216	+	1249	Protein_49	restriction endonuclease subunit S	-
AYR61_RS14495	39327..39671	-	345	WP_001748110.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYR61_RS14500	39665..39928	-	264	WP_001748109.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AYR61_RS14505	40014..40601	+	588	WP_065904085.1	site-specific integrase	-
AYR61_RS14510	40614..40979	+	366	WP_065904087.1	hypothetical protein	-
AYR61_RS14515	41325..41781	-	457	Protein_54	phage terminase small subunit P27 family	-
AYR61_RS14520	41974..42495	-	522	WP_065904088.1	HNH endonuclease	-
AYR61_RS14530	42976..43404	-	429	WP_065904091.1	transcriptional regulator	-
AYR61_RS14535	43448..43639	-	192	WP_065904095.1	hypothetical protein	-
AYR61_RS14540	43620..44060	-	441	WP_065904097.1	hypothetical protein	-
AYR61_RS14545	44060..44254	-	195	WP_065904100.1	hypothetical protein	-
AYR61_RS14550	44251..44613	-	363	WP_065904102.1	hypothetical protein	-
AYR61_RS15250	44606..44821	-	216	WP_191982309.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..50174	50174

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-104)

Antitoxin

• Antitoxin PemI-like (8-85)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13041.89 Da
• Isoelectric Point: 6.4782

>T61888 WP_001748110.1 NZ_CP014913:c39671-39327 [Secundilactobacillus paracollinoides]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Nucleotide

• Length: 345 bp

>T61888 NZ_CP014913:c39671-39327 [Secundilactobacillus paracollinoides]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTGGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10141.47 Da
• Isoelectric Point: 5.7117

>AT61888 WP_001748109.1 NZ_CP014913:c39928-39665 [Secundilactobacillus paracollinoides]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWQDDGKRDHELDWGKS
EGNELQW

Nucleotide

• Length: 264 bp

>AT61888 NZ_CP014913:c39928-39665 [Secundilactobacillus paracollinoides]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAGCTATTTAAGGATTGGCAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0R2K1X3

Antitoxin

• AlphaFold DB: A0A806J926