Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 9839..10440 Replicon plasmid pL1481-1
Accession NZ_CP014908
Organism Fructilactobacillus lindneri strain TMW 1.481

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag AYR59_RS07210 Protein ID WP_001748110.1
Coordinates 10096..10440 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0R2JU57
Locus tag AYR59_RS07205 Protein ID WP_054646951.1
Coordinates 9839..10102 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR59_RS07185 5895..6791 - 897 WP_078775231.1 glycosyl transferase -
AYR59_RS07190 6988..7923 - 936 WP_054646953.1 replication initiation protein -
AYR59_RS07195 8598..8810 - 213 WP_010015136.1 hypothetical protein -
AYR59_RS07200 9166..9753 - 588 WP_054646952.1 site-specific integrase -
AYR59_RS07205 9839..10102 + 264 WP_054646951.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AYR59_RS07210 10096..10440 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AYR59_RS07640 10674..10796 + 123 Protein_12 IS5/IS1182 family transposase -
AYR59_RS07645 10919..11350 - 432 WP_083210465.1 IS3 family transposase -
AYR59_RS07220 11424..11780 - 357 WP_065868735.1 fluoride efflux transporter CrcB -
AYR59_RS07225 11777..12178 - 402 WP_065868736.1 CrcB family protein -
AYR59_RS07235 13035..13544 + 510 WP_054646949.1 HTH domain-containing protein -
AYR59_RS07590 13553..13738 + 186 WP_156767909.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..14963 14963
- flank IS/Tn - - 10919..11350 431


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

SpoVT-AbrB domain

(12-55)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T61886 WP_001748110.1 NZ_CP014908:10096-10440 [Fructilactobacillus lindneri]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T61886 NZ_CP014908:10096-10440 [Fructilactobacillus lindneri]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAAATGAATAAGCGACCAGCCAT
TGCGCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10157.51 Da        Isoelectric Point: 6.4708

>AT61886 WP_054646951.1 NZ_CP014908:9839-10102 [Fructilactobacillus lindneri]
MELIKEQTRLSKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT61886 NZ_CP014908:9839-10102 [Fructilactobacillus lindneri]
ATGGAACTTATTAAAGAACAAACACGCTTATCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAAGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A0R2JU57

References