Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 1990..2591 Replicon plasmid pL12002-3
Accession NZ_CP014902
Organism Loigolactobacillus backii strain TMW 1.2002

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag AYR56_RS12595 Protein ID WP_001748110.1
Coordinates 1990..2334 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A192H634
Locus tag AYR56_RS12600 Protein ID WP_068281312.1
Coordinates 2328..2591 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR56_RS12590 9..1781 - 1773 WP_056947050.1 AIPR family protein -
AYR56_RS12595 1990..2334 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AYR56_RS12600 2328..2591 - 264 WP_068281312.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AYR56_RS12605 2677..3264 + 588 WP_068281313.1 site-specific integrase -
AYR56_RS12610 3620..3832 + 213 WP_068281314.1 hypothetical protein -
AYR56_RS12615 4552..5487 + 936 WP_002834578.1 replication initiation protein -
AYR56_RS12620 5480..5998 + 519 WP_068281315.1 HTH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..11878 11878


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T61884 WP_001748110.1 NZ_CP014902:c2334-1990 [Loigolactobacillus backii]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T61884 NZ_CP014902:c2334-1990 [Loigolactobacillus backii]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10154.51 Da        Isoelectric Point: 6.4708

>AT61884 WP_068281312.1 NZ_CP014902:c2591-2328 [Loigolactobacillus backii]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPNNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT61884 NZ_CP014902:c2591-2328 [Loigolactobacillus backii]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAAACAATATTCACGAACTATTTAAGGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A192H634

References