Detailed information of TA system TA061883

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 2432858..2433430
• Replicon: chromosome
• Accession: NZ_CP014899
• Organism: Loigolactobacillus backii strain TMW 1.2002

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A192H2Z7
• Locus tag: AYR56_RS11235
• Protein ID: WP_068281166.1
• Coordinates: 2432858..2433214 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: AYR56_RS11240
• Protein ID: WP_082918581.1
• Coordinates: 2433236..2433430 (-)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR56_RS11220	2429575..2430267	-	693	WP_068225551.1	O-methyltransferase	-
AYR56_RS11225	2430381..2431019	+	639	WP_068281165.1	CBS domain-containing protein	-
AYR56_RS11230	2431440..2432711	+	1272	WP_035457977.1	ISL3 family transposase	-
AYR56_RS11235	2432858..2433214	-	357	WP_068281166.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYR56_RS11240	2433236..2433430	-	195	WP_082918581.1	hypothetical protein	Antitoxin
AYR56_RS11245	2433594..2434703	-	1110	WP_068281167.1	alanine racemase	-
AYR56_RS11250	2434703..2435062	-	360	WP_068281168.1	holo-ACP synthase	-
AYR56_RS11255	2435257..2436810	-	1554	WP_068225557.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12726.89 Da
• Isoelectric Point: 9.9289

>T61883 WP_068281166.1 NZ_CP014899:c2433214-2432858 [Loigolactobacillus backii]
MDPVVKRGDIFYADLSPVVGSEQGGMRPVLVVQNNIGNHYSPTVIVAAITAKIQKPKMPTHVGITAKQTGIEKDSVILLE
QIRTIDKQRLRDKVAHLADDLMAKVDSAVAVSVGLKSI

Nucleotide

• Length: 357 bp

>T61883 NZ_CP014899:c2433214-2432858 [Loigolactobacillus backii]
ATGGATCCAGTTGTAAAACGCGGGGATATTTTTTATGCAGATCTTTCACCAGTCGTTGGTTCAGAACAAGGTGGAATGCG
ACCAGTATTAGTGGTCCAAAATAATATTGGTAATCATTACAGCCCAACTGTCATTGTTGCGGCGATTACTGCCAAGATCC
AAAAGCCCAAAATGCCAACACATGTAGGTATAACGGCCAAACAGACTGGCATTGAAAAAGATTCAGTAATCTTGTTAGAA
CAAATTAGAACAATTGACAAACAACGTCTACGCGATAAGGTGGCACATTTGGCTGATGATTTAATGGCCAAGGTTGATAG
TGCAGTTGCGGTAAGCGTAGGTCTTAAAAGTATTTAA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7449.37 Da
• Isoelectric Point: 3.9936

>AT61883 WP_082918581.1 NZ_CP014899:c2433430-2433236 [Loigolactobacillus backii]
IDAYCEYYSVDENDLVNGAMMEFFKIHQQKLDTLINGYIEMGHLNAEIAREFSPCECEADQLIR

Nucleotide

• Length: 195 bp

>AT61883 NZ_CP014899:c2433430-2433236 [Loigolactobacillus backii]
ATCGACGCATACTGTGAATATTATTCGGTGGATGAAAATGACTTAGTAAATGGTGCTATGATGGAATTCTTTAAAATCCA
CCAACAGAAACTTGACACTCTCATCAACGGTTATATTGAGATGGGACATTTAAACGCAGAGATTGCGCGTGAATTCAGTC
CGTGTGAATGTGAGGCGGATCAGCTCATCCGTTAG

Structures

Toxin

• AlphaFold DB: A0A192H2Z7