Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 9099..9700 Replicon plasmid pL11992-4
Accession NZ_CP014894
Organism Loigolactobacillus backii strain TMW 1.1992

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag AYR55_RS12630 Protein ID WP_001748110.1
Coordinates 9356..9700 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q684P2
Locus tag AYR55_RS12625 Protein ID WP_003643337.1
Coordinates 9099..9362 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR55_RS12600 4236..5852 + 1617 WP_068377466.1 DNA primase family protein -
AYR55_RS12605 6153..6566 + 414 WP_068377470.1 hypothetical protein -
AYR55_RS12610 6725..7183 - 459 Protein_9 phage terminase small subunit P27 family -
AYR55_RS12615 7674..7973 - 300 Protein_10 helix-turn-helix domain-containing protein -
AYR55_RS12620 8426..9013 - 588 WP_033609932.1 site-specific integrase -
AYR55_RS12625 9099..9362 + 264 WP_003643337.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AYR55_RS12630 9356..9700 + 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AYR55_RS12635 9810..11042 - 1233 WP_068377478.1 restriction endonuclease subunit S -
AYR55_RS12640 11032..12654 - 1623 WP_068377481.1 type I restriction-modification system subunit M -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..33159 33159


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T61882 WP_001748110.1 NZ_CP014894:9356-9700 [Loigolactobacillus backii]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T61882 NZ_CP014894:9356-9700 [Loigolactobacillus backii]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCTCTGAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.51 Da        Isoelectric Point: 6.4708

>AT61882 WP_003643337.1 NZ_CP014894:9099-9362 [Loigolactobacillus backii]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT61882 NZ_CP014894:9099-9362 [Loigolactobacillus backii]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L4ZZT2

References