Detailed information of TA system TA061881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 1759316..1759888
• Replicon: chromosome
• Accession: NZ_CP014890
• Organism: Loigolactobacillus backii strain TMW 1.1992

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A192H2Z7
• Locus tag: AYR55_RS08245
• Protein ID: WP_068281166.1
• Coordinates: 1759316..1759672 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: AYR55_RS08250
• Protein ID: WP_082918581.1
• Coordinates: 1759694..1759888 (-)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR55_RS08230	1754527..1757178	-	2652	WP_068225550.1	pyruvate, phosphate dikinase	-
AYR55_RS08235	1757509..1758201	-	693	WP_068225551.1	O-methyltransferase	-
AYR55_RS08240	1758315..1758953	+	639	WP_068225552.1	CBS domain-containing protein	-
AYR55_RS08245	1759316..1759672	-	357	WP_068281166.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYR55_RS08250	1759694..1759888	-	195	WP_082918581.1	hypothetical protein	Antitoxin
AYR55_RS08255	1760052..1761161	-	1110	WP_068225555.1	alanine racemase	-
AYR55_RS08260	1761161..1761520	-	360	WP_068225556.1	holo-ACP synthase	-
AYR55_RS08265	1761730..1762977	-	1248	WP_057808145.1	ISL3 family transposase	-
AYR55_RS08270	1763170..1764711	-	1542	WP_068377064.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12726.89 Da
• Isoelectric Point: 9.9289

>T61881 WP_068281166.1 NZ_CP014890:c1759672-1759316 [Loigolactobacillus backii]
MDPVVKRGDIFYADLSPVVGSEQGGMRPVLVVQNNIGNHYSPTVIVAAITAKIQKPKMPTHVGITAKQTGIEKDSVILLE
QIRTIDKQRLRDKVAHLADDLMAKVDSAVAVSVGLKSI

Nucleotide

• Length: 357 bp

>T61881 NZ_CP014890:c1759672-1759316 [Loigolactobacillus backii]
ATGGATCCAGTTGTAAAACGCGGGGATATTTTTTATGCAGATCTTTCACCAGTCGTTGGTTCAGAACAAGGTGGAATGCG
ACCAGTATTAGTGGTCCAAAATAATATTGGTAATCATTACAGCCCAACTGTCATTGTTGCGGCGATTACTGCCAAGATCC
AAAAGCCCAAAATGCCAACACATGTAGGTATAACGGCTAAACAGACTGGCATTGAAAAAGATTCAGTAATCTTGTTAGAA
CAAATTAGAACAATTGACAAACAACGTCTACGCGATAAGGTGGCACATTTGGCTGATGATTTAATGGCCAAGGTTGATAG
TGCAGTTGCGGTAAGCGTAGGTCTTAAAAGTATTTAA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7449.37 Da
• Isoelectric Point: 3.9936

>AT61881 WP_082918581.1 NZ_CP014890:c1759888-1759694 [Loigolactobacillus backii]
IDAYCEYYSVDENDLVNGAMMEFFKIHQQKLDTLINGYIEMGHLNAEIAREFSPCECEADQLIR

Nucleotide

• Length: 195 bp

>AT61881 NZ_CP014890:c1759888-1759694 [Loigolactobacillus backii]
ATCGACGCATACTGTGAATATTATTCGGTGGATGAAAATGACTTAGTAAATGGTGCTATGATGGAATTCTTTAAAATCCA
CCAACAGAAACTTGACACTCTCATCAACGGTTATATTGAGATGGGACATTTAAACGCAGAGATTGCGCGTGAATTCAGTC
CGTGTGAATGTGAGGCGGATCAGCTCATCCGTTAG

Structures

Toxin

• AlphaFold DB: A0A192H2Z7