Detailed information of TA system TA061879

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 1674496..1675068
• Replicon: chromosome
• Accession: NZ_CP014881
• Organism: Loigolactobacillus backii strain TMW 1.1991

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A192H2Z7
• Locus tag: AYR54_RS07655
• Protein ID: WP_068281166.1
• Coordinates: 1674496..1674852 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: AYR54_RS07660
• Protein ID: WP_082918581.1
• Coordinates: 1674874..1675068 (-)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR54_RS07640	1669707..1672358	-	2652	WP_068225550.1	pyruvate, phosphate dikinase	-
AYR54_RS07645	1672689..1673381	-	693	WP_068225551.1	O-methyltransferase	-
AYR54_RS07650	1673495..1674133	+	639	WP_068225552.1	CBS domain-containing protein	-
AYR54_RS07655	1674496..1674852	-	357	WP_068281166.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYR54_RS07660	1674874..1675068	-	195	WP_082918581.1	hypothetical protein	Antitoxin
AYR54_RS07665	1675232..1676341	-	1110	WP_068225555.1	alanine racemase	-
AYR54_RS07670	1676341..1676700	-	360	WP_068225556.1	holo-ACP synthase	-
AYR54_RS07675	1676910..1678157	-	1248	WP_057808145.1	ISL3 family transposase	-
AYR54_RS07680	1678350..1679903	-	1554	WP_068225557.1	DEAD/DEAH box helicase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12726.89 Da
• Isoelectric Point: 9.9289

>T61879 WP_068281166.1 NZ_CP014881:c1674852-1674496 [Loigolactobacillus backii]
MDPVVKRGDIFYADLSPVVGSEQGGMRPVLVVQNNIGNHYSPTVIVAAITAKIQKPKMPTHVGITAKQTGIEKDSVILLE
QIRTIDKQRLRDKVAHLADDLMAKVDSAVAVSVGLKSI

Nucleotide

• Length: 357 bp

>T61879 NZ_CP014881:c1674852-1674496 [Loigolactobacillus backii]
ATGGATCCAGTTGTAAAACGCGGGGATATTTTTTATGCAGATCTTTCACCAGTCGTTGGTTCAGAACAAGGTGGAATGCG
ACCAGTATTAGTGGTCCAAAATAATATTGGTAATCATTACAGCCCAACTGTCATTGTTGCGGCGATTACTGCCAAGATCC
AAAAGCCCAAAATGCCAACACATGTAGGTATAACGGCTAAACAGACTGGCATTGAAAAAGATTCAGTAATCTTGTTAGAA
CAAATTAGAACAATTGACAAACAACGTCTACGCGATAAGGTGGCACATTTGGCTGATGATTTAATGGCCAAGGTTGATAG
TGCAGTTGCGGTAAGCGTAGGTCTTAAAAGTATTTAA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7449.37 Da
• Isoelectric Point: 3.9936

>AT61879 WP_082918581.1 NZ_CP014881:c1675068-1674874 [Loigolactobacillus backii]
IDAYCEYYSVDENDLVNGAMMEFFKIHQQKLDTLINGYIEMGHLNAEIAREFSPCECEADQLIR

Nucleotide

• Length: 195 bp

>AT61879 NZ_CP014881:c1675068-1674874 [Loigolactobacillus backii]
ATCGACGCATACTGTGAATATTATTCGGTGGATGAAAATGACTTAGTAAATGGTGCTATGATGGAATTCTTTAAAATCCA
CCAACAGAAACTTGACACTCTCATCAACGGTTATATTGAGATGGGACATTTAAACGCAGAGATTGCGCGTGAATTCAGTC
CGTGTGAATGTGAGGCGGATCAGCTCATCCGTTAG

Structures

Toxin

• AlphaFold DB: A0A192H2Z7