Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09907-MazE
Location 2274..2875 Replicon plasmid pL11989-6
Accession NZ_CP014879
Organism Loigolactobacillus backii strain TMW 1.1989

Toxin (Protein)


Gene name pemK Uniprot ID Q684P1
Locus tag AYR53_RS13170 Protein ID WP_001748110.1
Coordinates 2274..2618 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID Q684P2
Locus tag AYR53_RS13175 Protein ID WP_003643337.1
Coordinates 2612..2875 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
AYR53_RS13155 512..715 + 204 WP_068281354.1 hypothetical protein -
AYR53_RS13160 784..1800 + 1017 WP_053359659.1 IS30 family transposase -
AYR53_RS13165 1847..2164 + 318 WP_156520662.1 hypothetical protein -
AYR53_RS13170 2274..2618 - 345 WP_001748110.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
AYR53_RS13175 2612..2875 - 264 WP_003643337.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
AYR53_RS13180 2961..3548 + 588 WP_068281356.1 site-specific integrase -
AYR53_RS13185 3830..5188 - 1359 WP_056973869.1 transposase -
AYR53_RS13190 6423..7352 + 930 WP_068281357.1 replication initiation protein -
AYR53_RS13195 7345..7857 + 513 WP_068281358.1 MarR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..12076 12076


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-104)

Antitoxin

Antitoxin PemI-like

(8-85)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13041.89 Da        Isoelectric Point: 6.4782

>T61877 WP_001748110.1 NZ_CP014879:c2618-2274 [Loigolactobacillus backii]
MVSQGDIFYVNFNPSRGHEQMNKRPAIALSNDLVCQTSNMTIVAPISSTKRNFPMYHRLTSSQTVYGKVLLDQTIALDLR
ARHVTDETIVDHVSREELEEIITLYKLLFSIDDK

Download         Length: 345 bp

>T61877 NZ_CP014879:c2618-2274 [Loigolactobacillus backii]
ATGGTAAGCCAAGGTGATATATTTTATGTTAACTTTAATCCAAGCCGTGGTCATGAACAGATGAATAAGCGACCAGCCAT
TGCTCTAAGCAATGATCTAGTCTGTCAGACAAGTAACATGACGATTGTGGCTCCAATTAGCAGTACCAAGCGTAATTTTC
CAATGTATCATCGACTAACTAGTAGTCAAACAGTATATGGAAAAGTATTATTAGATCAAACCATAGCCTTAGATTTACGG
GCAAGACATGTCACTGATGAAACTATTGTCGATCATGTATCGCGTGAAGAACTAGAAGAAATAATCACTTTGTACAAATT
ATTGTTTAGTATTGATGACAAATAG

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10141.51 Da        Isoelectric Point: 6.4708

>AT61877 WP_003643337.1 NZ_CP014879:c2875-2612 [Loigolactobacillus backii]
MELIKEQTRLAKWGNSKAARIPSQIIKQLKLDDNQDMTITIENGSIVLTPIKKNPTNIHELFKDWKDDGKRDHELDWGKS
EGNELQW

Download         Length: 264 bp

>AT61877 NZ_CP014879:c2875-2612 [Loigolactobacillus backii]
ATGGAACTTATTAAAGAACAAACACGCTTAGCAAAGTGGGGAAACTCGAAAGCTGCTAGAATTCCTAGCCAAATTATTAA
ACAACTGAAATTAGATGATAACCAAGATATGACAATAACCATTGAAAATGGATCAATTGTTTTAACACCAATAAAAAAGA
ACCCAACCAATATTCACGAACTATTTAAGGATTGGAAAGATGACGGGAAAAGGGATCACGAGCTTGATTGGGGAAAGTCA
GAAGGTAATGAACTTCAATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0R2K1X3

Antitoxin

Source ID Structure
AlphaFold DB A0A6L4ZZT2

References