Detailed information of TA system TA061876

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/MazF(toxin)
• Location: 1323080..1323652
• Replicon: chromosome
• Accession: NZ_CP014873
• Organism: Loigolactobacillus backii strain TMW 1.1989

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A192H2Z7
• Locus tag: AYR53_RS05750
• Protein ID: WP_068281166.1
• Coordinates: 1323296..1323652 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: AYR53_RS05745
• Protein ID: WP_082918581.1
• Coordinates: 1323080..1323274 (+)
• Length: 65 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR53_RS05730	1319700..1321253	+	1554	WP_068225557.1	DEAD/DEAH box helicase	-
AYR53_RS05735	1321448..1321807	+	360	WP_068281168.1	holo-ACP synthase	-
AYR53_RS05740	1321807..1322916	+	1110	WP_068281167.1	alanine racemase	-
AYR53_RS05745	1323080..1323274	+	195	WP_082918581.1	hypothetical protein	Antitoxin
AYR53_RS05750	1323296..1323652	+	357	WP_068281166.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AYR53_RS05755	1323799..1325070	-	1272	WP_035457977.1	ISL3 family transposase	-
AYR53_RS05760	1325491..1326129	-	639	WP_068281165.1	CBS domain-containing protein	-
AYR53_RS05765	1326243..1326935	+	693	WP_068225551.1	O-methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12726.89 Da
• Isoelectric Point: 9.9289

>T61876 WP_068281166.1 NZ_CP014873:1323296-1323652 [Loigolactobacillus backii]
MDPVVKRGDIFYADLSPVVGSEQGGMRPVLVVQNNIGNHYSPTVIVAAITAKIQKPKMPTHVGITAKQTGIEKDSVILLE
QIRTIDKQRLRDKVAHLADDLMAKVDSAVAVSVGLKSI

Nucleotide

• Length: 357 bp

>T61876 NZ_CP014873:1323296-1323652 [Loigolactobacillus backii]
ATGGATCCAGTTGTAAAACGCGGGGATATTTTTTATGCAGATCTTTCACCAGTCGTTGGTTCAGAACAAGGTGGAATGCG
ACCAGTATTAGTGGTCCAAAATAATATTGGTAATCATTACAGCCCAACTGTCATTGTTGCGGCGATTACTGCCAAGATCC
AAAAGCCCAAAATGCCAACACATGTAGGTATAACGGCCAAACAGACTGGCATTGAAAAAGATTCAGTAATCTTGTTAGAA
CAAATTAGAACAATTGACAAACAACGTCTACGCGATAAGGTGGCACATTTGGCTGATGATTTAATGGCCAAGGTTGATAG
TGCAGTTGCGGTAAGCGTAGGTCTTAAAAGTATTTAA

Antitoxin

Protein

• Length: 65 a.a.
• Molecular weight: 7449.37 Da
• Isoelectric Point: 3.9936

>AT61876 WP_082918581.1 NZ_CP014873:1323080-1323274 [Loigolactobacillus backii]
IDAYCEYYSVDENDLVNGAMMEFFKIHQQKLDTLINGYIEMGHLNAEIAREFSPCECEADQLIR

Nucleotide

• Length: 195 bp

>AT61876 NZ_CP014873:1323080-1323274 [Loigolactobacillus backii]
ATCGACGCATACTGTGAATATTATTCGGTGGATGAAAATGACTTAGTAAATGGTGCTATGATGGAATTCTTTAAAATCCA
CCAACAGAAACTTGACACTCTCATCAACGGTTATATTGAGATGGGACATTTAAACGCAGAGATTGCGCGTGAATTCAGTC
CGTGTGAATGTGAGGCGGATCAGCTCATCCGTTAG

Structures

Toxin

• AlphaFold DB: A0A192H2Z7