Detailed information of TA system TA061874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 6063482..6064013
• Replicon: chromosome
• Accession: NZ_CP014870
• Organism: Pseudomonas silesiensis strain A3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A191Z099
• Locus tag: PMA3_RS26855
• Protein ID: WP_064680005.1
• Coordinates: 6063711..6064013 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A6L5BNJ0
• Locus tag: PMA3_RS26850
• Protein ID: WP_054616768.1
• Coordinates: 6063482..6063721 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PMA3_RS26840	6059134..6059826	-	693	WP_064680003.1	DUF4391 domain-containing protein	-
PMA3_RS26845	6059823..6063113	-	3291	WP_064680004.1	DEAD/DEAH box helicase family protein	-
PMA3_RS26850	6063482..6063721	+	240	WP_054616768.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
PMA3_RS26855	6063711..6064013	+	303	WP_064680005.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PMA3_RS32745	6064017..6064109	+	93	Protein_5372	hypothetical protein	-
PMA3_RS26860	6064192..6065007	-	816	WP_064680006.1	DUF2135 domain-containing protein	-
PMA3_RS26865	6065011..6066630	-	1620	WP_064680007.1	DUF2300 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	6033437..6064013	30576

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11699.51 Da
• Isoelectric Point: 10.0983

>T61874 WP_064680005.1 NZ_CP014870:6063711-6064013 [Pseudomonas silesiensis]
MTYSLEFDRRALKEWNKLGGTLRQQFKKKLVEVLKNPRVEANRLRNLPDCYKIKLRSAGYRLIYQVIDQEVVVFVIAIGK
REGEAAYQDAQDRISQQPAD

Nucleotide

• Length: 303 bp

>T61874 NZ_CP014870:6063711-6064013 [Pseudomonas silesiensis]
ATGACCTATAGCCTCGAATTTGATCGTCGCGCATTGAAAGAGTGGAACAAACTGGGCGGCACCTTGCGCCAGCAGTTCAA
AAAGAAGCTTGTTGAGGTGTTGAAAAACCCTCGCGTTGAAGCCAACCGACTTCGTAATTTGCCAGATTGCTACAAGATAA
AGCTACGCAGTGCCGGCTATCGGCTGATCTATCAAGTGATTGATCAAGAGGTTGTGGTGTTTGTGATCGCCATTGGAAAG
CGAGAAGGTGAAGCCGCTTATCAAGATGCTCAGGACCGAATCAGTCAGCAACCTGCTGACTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8837.19 Da
• Isoelectric Point: 4.6017

>AT61874 WP_054616768.1 NZ_CP014870:6063482-6063721 [Pseudomonas silesiensis]
MASINIRIDDDLKQRSFAELEKLGVTPSELLRQTLQYVAERGKLPFKAALLSEEDEALIAVVTERLAAPQRVKVSLDDL

Nucleotide

• Length: 240 bp

>AT61874 NZ_CP014870:6063482-6063721 [Pseudomonas silesiensis]
ATGGCTTCTATCAACATTCGCATTGACGATGACCTGAAGCAGCGTTCATTTGCCGAGCTGGAAAAACTCGGCGTAACGCC
CTCCGAGCTTCTGCGCCAAACACTCCAATACGTCGCCGAACGAGGCAAATTGCCTTTCAAGGCAGCATTACTTAGCGAAG
AGGATGAAGCCCTCATTGCAGTGGTCACCGAGCGCCTTGCCGCCCCTCAGCGAGTCAAGGTTAGCCTGGATGACCTATAG

Structures

Toxin

• AlphaFold DB: A0A191Z099

Antitoxin

• AlphaFold DB: A0A6L5BNJ0