Detailed information of TA system TA061869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 1935898..1936775
• Replicon: chromosome
• Accession: NZ_CP014870
• Organism: Pseudomonas silesiensis strain A3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: PMA3_RS08770
• Protein ID: WP_064676789.1
• Coordinates: 1935898..1936251 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A191YQR7
• Locus tag: PMA3_RS08775
• Protein ID: WP_064676790.1
• Coordinates: 1936302..1936775 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PMA3_RS08750	1931752..1932933	+	1182	WP_064676785.1	SpoIIE family protein phosphatase	-
PMA3_RS08755	1932933..1933415	+	483	WP_064676786.1	STAS domain-containing protein	-
PMA3_RS08760	1933622..1934548	-	927	WP_064676787.1	transaldolase	-
PMA3_RS08765	1934727..1935713	-	987	WP_064676788.1	tRNA dihydrouridine(20/20a) synthase DusA	-
PMA3_RS08770	1935898..1936251	+	354	WP_064676789.1	type II toxin-antitoxin system HigB family toxin	Toxin
PMA3_RS08775	1936302..1936775	+	474	WP_064676790.1	hypothetical protein	Antitoxin
PMA3_RS08780	1936869..1937294	-	426	WP_064676791.1	DUF2628 domain-containing protein	-
PMA3_RS08785	1937649..1938056	+	408	WP_082930279.1	hypothetical protein	-
PMA3_RS08790	1938130..1939152	-	1023	WP_064676792.1	transketolase family protein	-
PMA3_RS08795	1939149..1939997	-	849	WP_064676793.1	transketolase	-
PMA3_RS08800	1940016..1941314	-	1299	WP_064676794.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (97-152)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13698.83 Da
• Isoelectric Point: 9.9869

>T61869 WP_064676789.1 NZ_CP014870:1935898-1936251 [Pseudomonas silesiensis]
MRVIAKAAVTQAMQKYSQWHAALSLWLTTFDKPGLRFESFEQTRNLWSATSGWNVDRVPRSKLRESSRKGPLDIYIFDIK
KNECRIICWINTMNGTIYIKNILPHAGYDKWCKSEVE

Nucleotide

• Length: 354 bp

>T61869 NZ_CP014870:1935898-1936251 [Pseudomonas silesiensis]
ATGCGGGTAATAGCGAAAGCGGCGGTCACGCAAGCCATGCAGAAATACAGTCAGTGGCACGCAGCGCTGAGTCTATGGCT
AACGACGTTTGATAAGCCAGGCCTGCGCTTCGAGTCGTTTGAGCAGACTCGAAACCTTTGGTCCGCCACGTCTGGATGGA
ACGTTGATCGAGTCCCTCGCTCAAAACTTCGGGAGTCGAGCAGGAAGGGGCCCCTTGATATTTATATTTTTGATATCAAG
AAGAATGAGTGCCGGATTATTTGTTGGATAAATACGATGAATGGCACCATTTATATTAAAAACATCCTGCCTCACGCCGG
ATACGACAAGTGGTGTAAAAGTGAGGTCGAGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17572.06 Da
• Isoelectric Point: 4.8632

>AT61869 WP_064676790.1 NZ_CP014870:1936302-1936775 [Pseudomonas silesiensis]
MKRDKIEATGLDAFIANISPMLDGLSDQMATMSRLLSACHEKIKDDKDLQGRMALIDELYSHADSEGHVAARFAELVADR
VYEYETETVLIPYSSQSEALAFLIADRGVKQKDLSEIASQSAISEMLNNKRKMTVSQIKGFSDYFKVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT61869 NZ_CP014870:1936302-1936775 [Pseudomonas silesiensis]
ATGAAGCGTGATAAAATCGAAGCCACTGGACTAGATGCCTTCATAGCTAATATTTCACCGATGCTTGATGGTCTTAGCGA
TCAGATGGCGACCATGTCCCGGTTATTGTCTGCCTGTCATGAAAAAATCAAGGATGATAAAGACTTACAGGGGCGCATGG
CGCTAATCGATGAGCTTTACTCTCATGCTGATAGTGAAGGGCATGTAGCAGCACGCTTCGCGGAGTTGGTGGCTGATCGT
GTTTATGAGTACGAGACTGAGACGGTTTTGATCCCTTACTCCTCTCAATCGGAAGCGTTGGCGTTTCTTATTGCCGATAG
GGGAGTTAAACAAAAGGACTTGTCCGAAATTGCTTCTCAAAGTGCCATTTCAGAAATGCTGAACAATAAAAGAAAAATGA
CTGTTTCTCAGATAAAAGGCTTTTCTGATTACTTTAAGGTTCCTGTCGAGTTTTTTATGCACGGTGTAGTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A191YQR7