Detailed information of TA system TA061867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 3716566..3717155
• Replicon: chromosome
• Accession: NZ_CP014867
• Organism: Pseudomonas chlororaphis strain 189 isolate 189

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A285IHJ3
• Locus tag: A3218_RS16310
• Protein ID: WP_062824477.1
• Coordinates: 3716566..3716865 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: A3218_RS16315
• Protein ID: WP_007924096.1
• Coordinates: 3716862..3717155 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3218_RS16275	3712137..3712379	-	243	WP_007924102.1	exodeoxyribonuclease VII small subunit	-
A3218_RS16280	3712617..3712772	+	156	WP_155736162.1	hypothetical protein	-
A3218_RS16285	3712870..3713112	+	243	WP_062824474.1	hypothetical protein	-
A3218_RS16290	3713397..3714485	+	1089	WP_062824475.1	nitrite reductase, copper-containing	-
A3218_RS16295	3714553..3714852	+	300	WP_007924100.1	ArsR family transcriptional regulator	-
A3218_RS16300	3714910..3715920	+	1011	WP_202908065.1	NAD(P)-dependent alcohol dehydrogenase	-
A3218_RS16305	3715945..3716394	-	450	WP_009045812.1	DUF3828 domain-containing protein	-
A3218_RS16310	3716566..3716865	+	300	WP_062824477.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A3218_RS16315	3716862..3717155	+	294	WP_007924096.1	putative addiction module antidote protein	Antitoxin
A3218_RS16320	3717271..3717657	-	387	WP_062824478.1	hypothetical protein	-
A3218_RS16325	3718440..3718967	-	528	WP_062824479.1	inorganic diphosphatase	-
A3218_RS16330	3719232..3720041	-	810	WP_062824480.1	zinc-dependent peptidase	-
A3218_RS16335	3720047..3720703	-	657	WP_007924093.1	DedA family protein	-
A3218_RS16340	3720953..3721411	-	459	WP_009045805.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (6-91)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11261.01 Da
• Isoelectric Point: 10.8652

>T61867 WP_062824477.1 NZ_CP014867:3716566-3716865 [Pseudomonas chlororaphis]
MIEIKQTATFMEWENRLKDSRARALIAARIFRLANGLAGDVSPVGEGVSELRIHYGPGYRVYFQQRGLQLVILLCGGDKS
RQGRDIELAKQLARRWSRS

Nucleotide

• Length: 300 bp

>T61867 NZ_CP014867:3716566-3716865 [Pseudomonas chlororaphis]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATTGC
TGCGCGGATTTTTCGCCTGGCCAACGGTCTTGCCGGGGACGTGTCGCCGGTGGGCGAAGGCGTCAGCGAGCTGCGCATCC
ATTACGGGCCGGGCTACCGGGTTTACTTCCAGCAGCGCGGCTTGCAACTCGTCATTCTGTTGTGCGGCGGCGACAAGAGT
CGCCAGGGCCGCGATATCGAACTGGCCAAACAGCTGGCCCGTCGTTGGAGCCGATCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10430.94 Da
• Isoelectric Point: 5.6558

>AT61867 WP_007924096.1 NZ_CP014867:3716862-3717155 [Pseudomonas chlororaphis]
MNEPLFDYDPATALRDPQAIALFMADAFETGDAGYIAKALGVVARAKGMTELAHETGLSREQLYRSFSDKGNPTLKTLLA
VMRALGLDMTARVHGAS

Nucleotide

• Length: 294 bp

>AT61867 NZ_CP014867:3716862-3717155 [Pseudomonas chlororaphis]
ATGAACGAACCGCTTTTCGACTATGACCCCGCCACGGCCCTGCGTGACCCGCAAGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGCATGACCGAACTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTACCGCTCATTCAGCGACAAGGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGTGCACTGGGGCTGGACATGACGGCGCGGGTGCATGGGGCGTCTTGA

Structures

Toxin

• AlphaFold DB: A0A285IHJ3