Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/HicA-HicB
Location 1417205..1417827 Replicon chromosome
Accession NZ_CP014867
Organism Pseudomonas chlororaphis strain 189 isolate 189

Toxin (Protein)


Gene name hicA Uniprot ID A0A285HEJ8
Locus tag A3218_RS05920 Protein ID WP_053259927.1
Coordinates 1417205..1417387 (+) Length 61 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A285HET1
Locus tag A3218_RS05925 Protein ID WP_009042564.1
Coordinates 1417420..1417827 (+) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A3218_RS31735 1412629..1412934 + 306 WP_155736144.1 hypothetical protein -
A3218_RS05900 1413491..1413976 + 486 WP_202908048.1 sn-glycerol-3-phosphate transporter -
A3218_RS30820 1414775..1415218 + 444 WP_081232238.1 response regulator -
A3218_RS05910 1415287..1415871 - 585 WP_155736145.1 response regulator transcription factor -
A3218_RS05915 1416411..1416832 - 422 Protein_1192 DDE-type integrase/transposase/recombinase -
A3218_RS05920 1417205..1417387 + 183 WP_053259927.1 type II toxin-antitoxin system HicA family toxin Toxin
A3218_RS05925 1417420..1417827 + 408 WP_009042564.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
A3218_RS05930 1418033..1418947 + 915 WP_062823417.1 SGNH/GDSL hydrolase family protein -
A3218_RS05935 1418981..1420759 - 1779 WP_062823418.1 sensor domain-containing phosphodiesterase -
A3218_RS05940 1420998..1421888 - 891 WP_062826205.1 LysR family transcriptional regulator -
A3218_RS05945 1421985..1422653 + 669 WP_155736146.1 NAD(P)-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1416496..1416606 110


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(6-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-128)


Sequences

Toxin        


Download         Length: 61 a.a.        Molecular weight: 6873.18 Da        Isoelectric Point: 11.1084

>T61862 WP_053259927.1 NZ_CP014867:1417205-1417387 [Pseudomonas chlororaphis]
VDSRYLIGQIVADGWYLVRVRGSHHHFKHPHKPGLVTVPHPKKDLLRKTAISILKQALLM

Download         Length: 183 bp

>T61862 NZ_CP014867:1417205-1417387 [Pseudomonas chlororaphis]
GTGGACAGTCGGTATTTGATCGGCCAAATCGTCGCAGACGGTTGGTATCTGGTCAGGGTCAGGGGCAGTCACCACCATTT
CAAGCATCCCCATAAACCGGGGCTGGTGACCGTTCCCCATCCCAAAAAGGACCTGCTGAGAAAAACCGCCATCAGCATTC
TGAAACAGGCGCTGCTCATGTGA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14698.72 Da        Isoelectric Point: 4.5911

>AT61862 WP_009042564.1 NZ_CP014867:1417420-1417827 [Pseudomonas chlororaphis]
MLYPIAISMGDDEHAWGVEVPDIPGCFSAGDDLDDAMAMAREAIEGHFEILAEDGAPIPHANKVTLHAANPRYAGCTWAL
IDIDVTKYLGKAQKLNITLPGYLLNRIDEYVLHHPEAKSRSGFLASAALKVLQQD

Download         Length: 408 bp

>AT61862 NZ_CP014867:1417420-1417827 [Pseudomonas chlororaphis]
ATGCTTTACCCGATTGCAATTTCAATGGGCGATGACGAACACGCCTGGGGGGTGGAAGTCCCCGACATTCCTGGCTGTTT
CTCGGCCGGCGACGACCTCGACGACGCCATGGCCATGGCCCGCGAAGCCATCGAAGGGCACTTCGAGATTCTCGCCGAAG
ACGGTGCCCCCATTCCGCACGCCAACAAGGTTACCCTGCACGCGGCCAACCCCCGGTACGCAGGCTGCACCTGGGCGCTG
ATCGACATCGATGTCACCAAATACCTGGGCAAGGCGCAAAAGCTCAACATCACCCTGCCCGGCTACTTGCTGAACCGCAT
CGACGAATACGTGCTGCATCACCCGGAAGCCAAGAGCCGCTCGGGTTTTCTCGCTTCAGCTGCACTGAAGGTATTGCAAC
AAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A285HEJ8

Antitoxin

Source ID Structure
AlphaFold DB A0A285HET1

References