Detailed information of TA system TA061859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 371435..372020
• Replicon: chromosome
• Accession: NZ_CP014867
• Organism: Pseudomonas chlororaphis strain 189 isolate 189

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: A3218_RS01435
• Protein ID: WP_062822913.1
• Coordinates: 371435..371728 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A3G7H967
• Locus tag: A3218_RS01440
• Protein ID: WP_062822914.1
• Coordinates: 371730..372020 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3218_RS01415	366451..366846	-	396	WP_062822909.1	carboxymuconolactone decarboxylase family protein	-
A3218_RS01420	367078..368322	-	1245	WP_062822910.1	M20/M25/M40 family metallo-hydrolase	-
A3218_RS01425	368416..369540	-	1125	WP_062822911.1	diguanylate cyclase	-
A3218_RS01430	369805..371199	+	1395	WP_062822912.1	VOC family protein	-
A3218_RS01435	371435..371728	+	294	WP_062822913.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
A3218_RS01440	371730..372020	+	291	WP_062822914.1	putative addiction module antidote protein	Antitoxin
A3218_RS01445	372416..373003	-	588	WP_062822915.1	hypothetical protein	-
A3218_RS01450	373122..374504	-	1383	WP_062822916.1	efflux transporter outer membrane subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-96)

Antitoxin

• Uncharacterized protein HI1420 (11-94)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10743.39 Da
• Isoelectric Point: 10.0959

>T61859 WP_062822913.1 NZ_CP014867:371435-371728 [Pseudomonas chlororaphis]
MDYEILQTTVFAQWHTTLRDLRARIAIARRIERASAGNLGDVKNLGGGLSEMRVNMGAGYRIYFTVRGNTLIVLLLGGDK
STQPADICQARSLAKEF

Nucleotide

• Length: 294 bp

>T61859 NZ_CP014867:371435-371728 [Pseudomonas chlororaphis]
ATGGATTACGAGATCCTGCAGACGACCGTCTTTGCCCAATGGCACACCACGCTACGGGACCTGCGCGCCAGGATAGCCAT
CGCTCGGCGTATCGAGCGGGCTTCGGCGGGCAACCTGGGCGATGTGAAAAACCTCGGCGGCGGTCTCTCGGAAATGCGCG
TGAATATGGGAGCCGGCTACCGCATCTATTTCACCGTGCGCGGTAATACCCTCATCGTCCTGCTGCTGGGCGGCGACAAG
TCTACCCAGCCTGCCGATATCTGCCAGGCCCGTTCCCTGGCCAAGGAGTTCTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10253.80 Da
• Isoelectric Point: 8.0096

>AT61859 WP_062822914.1 NZ_CP014867:371730-372020 [Pseudomonas chlororaphis]
MSDTLLPFDIATLLDSDQAISEYLSQVLADGDTDELIRALGHVARARGMTQIAKATGQGRASLYKAFAPGAKPRFDTVVK
VMRALGLQLCTTVSRG

Nucleotide

• Length: 291 bp

>AT61859 NZ_CP014867:371730-372020 [Pseudomonas chlororaphis]
ATGTCCGACACCCTGCTTCCCTTCGACATCGCCACCCTGCTCGACAGCGACCAGGCGATCAGCGAGTACCTGTCCCAGGT
CCTGGCCGATGGCGATACCGATGAACTGATCCGCGCCCTGGGCCATGTCGCCAGGGCGCGGGGCATGACGCAGATTGCCA
AGGCCACCGGCCAGGGCCGGGCCAGCCTCTACAAGGCTTTTGCCCCGGGAGCCAAGCCGCGTTTCGATACCGTGGTCAAG
GTCATGCGCGCGCTGGGCCTGCAGCTGTGCACCACCGTGTCCCGCGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A3G7H967