Detailed information of TA system TA061851

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): abiEi-abiEii/AbiEii(toxin)
• Location: 13409..15038
• Replicon: plasmid unnamed
• Accession: NZ_CP014863
• Organism: Thermococcus profundus strain DT 5432

Toxin (Protein)

• Gene name: PygT
• Uniprot ID: -
• Locus tag: A3L09_RS10680
• Protein ID: WP_088859067.1
• Coordinates: 14022..15038 (+)
• Length: 339 a.a.

Antitoxin (Protein)

• Gene name: PygA
• Uniprot ID: -
• Locus tag: A3L09_RS10675
• Protein ID: WP_088859091.1
• Coordinates: 13409..14032 (+)
• Length: 208 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A3L09_RS10665 (A3L09_10630)	10838..12643	+	1806	WP_088859065.1	hypothetical protein	-
A3L09_RS10675 (A3L09_10640)	13409..14032	+	624	WP_088859091.1	hypothetical protein	Antitoxin
A3L09_RS10680 (A3L09_10645)	14022..15038	+	1017	WP_088859067.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
A3L09_RS10685 (A3L09_10650)	15031..18411	+	3381	WP_088859068.1	helicase-related protein	-
A3L09_RS10690 (A3L09_10655)	18467..18925	+	459	WP_232473654.1	DUF365 domain-containing protein	-
A3L09_RS10695 (A3L09_10660)	19006..19398	+	393	WP_088859070.1	hypothetical protein	-
A3L09_RS10700 (A3L09_10665)	19424..19774	-	351	WP_088859071.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38045	38045

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (46-278)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 339 a.a.
• Molecular weight: 39940.81 Da
• Isoelectric Point: 7.7850

>T61851 WP_088859067.1 NZ_CP014863:14022-15038 [Thermococcus profundus]
MEGNSLLMEIRRNERRGFARFVSRKTGIKSIDLVEWDYIIHTILKELERDPQFKENYIFKGGTCLVKCHLGYYRFSRDLD
FAYRDSDRLRELSRSQLRQFLNEETGRIAEILKCVAEDLGLEFQYSGHNDFNNHRYFSFRIGPGWFREIVLYSPLGEKIK
IEVNYAEKLAFKPKTLKANTLLSWKGITLTSKEYEKYIEFLGNYYPLRILAYSDREILVEKVRALLTRREFKLRDLYDLY
KLNQRGLEISKYKNKIVDKIRNYLALSSLAREHLEVALNSIEEGTFLTDIGAEIERDSGLIVEPFSKEEFLKFVDELREE
LMEISGDLKPLNEVKNNG

Nucleotide

• Length: 1017 bp

>T61851 NZ_CP014863:14022-15038 [Thermococcus profundus]
ATGGAAGGTAATTCACTCCTGATGGAAATCCGCAGGAACGAAAGGAGAGGTTTTGCAAGGTTCGTTTCCAGAAAAACAGG
AATAAAATCCATTGATCTCGTCGAATGGGACTACATAATCCATACGATTTTGAAAGAACTTGAGAGGGATCCGCAGTTTA
AGGAAAACTACATTTTCAAAGGTGGCACATGCCTTGTGAAGTGTCACCTCGGTTATTACCGCTTCAGCAGAGATCTGGAT
TTCGCATACCGGGATAGCGACAGGCTTAGAGAACTGAGCAGGAGTCAACTCAGGCAGTTCCTGAACGAGGAAACGGGAAG
AATAGCAGAAATCCTGAAGTGCGTCGCGGAGGACTTAGGCCTTGAGTTTCAGTACTCGGGGCATAATGACTTCAATAACC
ATAGGTATTTTAGCTTCAGGATTGGTCCGGGCTGGTTCAGGGAGATAGTGTTGTACTCCCCACTCGGTGAGAAGATAAAA
ATAGAAGTAAACTACGCGGAAAAACTCGCGTTTAAGCCTAAAACATTGAAAGCGAACACATTATTATCCTGGAAGGGGAT
TACGCTCACTTCAAAGGAATATGAAAAGTACATAGAATTCCTTGGCAACTATTATCCTCTTCGCATTCTTGCATACTCTG
ACAGGGAAATCCTCGTGGAGAAAGTCAGGGCACTCTTAACCAGACGGGAGTTTAAGCTCAGGGACCTCTATGATTTGTAC
AAACTCAATCAACGTGGGCTTGAGATTTCAAAATACAAGAATAAAATAGTCGACAAGATAAGAAACTACTTGGCCTTGAG
TTCGTTAGCAAGGGAACATCTGGAAGTTGCTCTGAATTCAATTGAAGAAGGCACTTTCCTCACAGATATTGGCGCCGAGA
TAGAGAGGGATAGTGGCCTGATTGTGGAACCTTTCAGCAAAGAAGAATTTTTGAAGTTTGTTGATGAGTTAAGAGAAGAA
TTAATGGAAATCTCAGGAGATTTAAAACCTCTAAATGAGGTGAAAAACAATGGCTGA

Antitoxin

Protein

• Length: 208 a.a.
• Molecular weight: 24185.05 Da
• Isoelectric Point: 9.8678

>AT61851 WP_088859091.1 NZ_CP014863:13409-14032 [Thermococcus profundus]
MKPIIQLLLSDYGGQVITREELEKLATYFSEDVDYLVNYLIQYGYVIRILRGLYYVKTPLEFSSGGSPPVYKLLSLGMSR
ITENWYFGLFTALVLNGLTHETFTTVFVINDRIARPKVINVNGTPVRIIRSRRNIFDFGIIVRDSLKLSDIEKTLLDFLY
FSSYGTVSRELALRIWREYSGYADREKLKRYSERYPMSVRKVIRDGR

Nucleotide

• Length: 624 bp

>AT61851 NZ_CP014863:13409-14032 [Thermococcus profundus]
ATGAAGCCGATCATTCAGCTGCTCCTTTCGGATTACGGTGGACAGGTAATAACCCGCGAAGAACTGGAGAAGCTCGCCAC
TTACTTCAGCGAGGACGTTGACTATCTCGTGAACTACCTTATCCAGTACGGCTACGTTATTAGAATTCTCCGGGGTTTGT
ACTACGTGAAAACTCCGCTGGAATTCTCATCGGGGGGTTCCCCACCAGTCTACAAACTCCTCTCTCTCGGAATGAGCAGG
ATAACGGAGAACTGGTATTTCGGTCTGTTTACGGCCCTGGTACTCAACGGCCTCACCCACGAGACATTCACAACCGTTTT
CGTTATCAACGACAGAATAGCCAGGCCAAAAGTCATAAACGTGAACGGAACACCCGTGAGAATAATCAGGAGCAGGCGGA
ATATCTTCGATTTCGGAATAATCGTAAGGGACTCGTTGAAGCTCTCCGACATTGAAAAGACCCTTCTTGATTTCCTCTAC
TTTTCCAGTTATGGTACTGTTTCCAGGGAACTTGCCCTGAGAATCTGGAGGGAATACTCGGGGTATGCTGATAGAGAAAA
GCTCAAACGATATTCCGAGAGGTATCCAATGTCAGTGAGGAAGGTGATCCGGGATGGAAGGTAA