Detailed information of TA system TA061848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3587048..3587583
• Replicon: chromosome
• Accession: NZ_CP014860
• Organism: Mycobacterium shottsii strain M175

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: TM48_RS16565
• Protein ID: WP_198965432.1
• Coordinates: 3587251..3587583 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7I7LGS6
• Locus tag: TM48_RS16560
• Protein ID: WP_036455700.1
• Coordinates: 3587048..3587254 (+)
• Length: 69 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TM48_RS16540 (TM48_03295)	3582517..3583182	+	666	WP_231608608.1	ParA family protein	-
TM48_RS16545 (TM48_03296)	3583275..3584102	-	828	WP_020729183.1	DUF1906 domain-containing protein	-
TM48_RS16550 (TM48_03297)	3584430..3586116	-	1687	Protein_3260	GMC family oxidoreductase N-terminal domain-containing protein	-
TM48_RS16555 (TM48_03298)	3586365..3586715	-	351	WP_198965431.1	cupin domain-containing protein	-
TM48_RS16560 (TM48_03299)	3587048..3587254	+	207	WP_036455700.1	DUF3018 family protein	Antitoxin
TM48_RS16565 (TM48_03300)	3587251..3587583	+	333	WP_198965432.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
TM48_RS16570	3588364..3588531	+	168	WP_259594304.1	MbcA/ParS/Xre antitoxin family protein	-
TM48_RS16575 (TM48_03302)	3588532..3589086	+	555	WP_020729188.1	RES domain-containing protein	-
TM48_RS16580 (TM48_03303)	3589364..3589597	+	234	WP_020729189.1	hypothetical protein	-
TM48_RS16585 (TM48_03304)	3589587..3589898	+	312	WP_020729190.1	type II toxin-antitoxin system PemK/MazF family toxin	-
TM48_RS16590 (TM48_03305)	3590151..3590876	-	726	WP_198968070.1	hypothetical protein	-
TM48_RS16595 (TM48_03307)	3590875..3591947	+	1073	Protein_3269	IS3-like element ISMyma1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-105)

Antitoxin

• Antitoxin MazE-like (4-66)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11899.70 Da
• Isoelectric Point: 6.7245

>T61848 WP_198965432.1 NZ_CP014860:3587251-3587583 [Mycobacterium shottsii]
VNRGEIWTVAGDVYATKPHPAVIVQDDLFDATSSVTVAPMSSTLLDAPLMRIRIAGGDGRLSGLDHNSDVMIDKLTTVKR
SNVHARVGRLTAEQVVEVERVMMAFLGLAR

Nucleotide

• Length: 333 bp

>T61848 NZ_CP014860:3587251-3587583 [Mycobacterium shottsii]
GTGAATAGAGGGGAGATCTGGACCGTAGCGGGGGACGTCTACGCGACCAAACCGCATCCCGCGGTGATTGTTCAGGATGA
TCTCTTTGATGCCACGAGTTCGGTGACGGTTGCTCCGATGTCCAGCACCCTGTTGGATGCGCCGTTGATGCGCATCCGAA
TAGCCGGCGGAGACGGCCGGTTATCCGGACTTGATCACAACAGTGACGTGATGATCGACAAGCTCACAACCGTGAAAAGG
TCGAACGTCCATGCCCGAGTCGGTCGGCTGACAGCGGAGCAAGTCGTCGAGGTCGAGCGAGTGATGATGGCATTCCTCGG
CCTTGCGCGATAG

Antitoxin

Protein

• Length: 69 a.a.
• Molecular weight: 7999.86 Da
• Isoelectric Point: 4.8359

>AT61848 WP_036455700.1 NZ_CP014860:3587048-3587254 [Mycobacterium shottsii]
MAVRDRVGEYRRRMRERGLRPLQVWVPDVRTESFAAEAHRQASLVARADERGDDQDFIEAISTPWDEE

Nucleotide

• Length: 207 bp

>AT61848 NZ_CP014860:3587048-3587254 [Mycobacterium shottsii]
ATGGCGGTTAGGGACAGGGTCGGCGAGTACCGACGGCGGATGCGGGAGCGGGGCCTGCGGCCGCTGCAGGTCTGGGTGCC
TGACGTGCGCACTGAGAGTTTTGCTGCCGAGGCGCATCGTCAGGCTTCGTTGGTCGCACGAGCGGACGAACGCGGCGATG
ATCAGGACTTCATTGAGGCCATCTCGACGCCATGGGATGAGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7LGS6