Detailed information of TA system TA061847

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1498820..1499459
• Replicon: chromosome
• Accession: NZ_CP014860
• Organism: Mycobacterium shottsii strain M175

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: TM48_RS07110
• Protein ID: WP_198967264.1
• Coordinates: 1499013..1499459 (+)
• Length: 149 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: B2HEJ8
• Locus tag: TM48_RS07105
• Protein ID: WP_011738542.1
• Coordinates: 1498820..1499008 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
TM48_RS07085	1494548..1495330	+	783	WP_259594278.1	MFS transporter	-
TM48_RS07090 (TM48_01403)	1495389..1496506	+	1118	Protein_1391	IS3-like element ISMyma1 family transposase	-
TM48_RS07095 (TM48_01404)	1496503..1496937	+	435	WP_198967262.1	pentapeptide repeat-containing protein	-
TM48_RS07100 (TM48_01405)	1496895..1498718	+	1824	WP_198967263.1	PPE family protein	-
TM48_RS07105 (TM48_01406)	1498820..1499008	+	189	WP_011738542.1	antitoxin	Antitoxin
TM48_RS07110 (TM48_01407)	1499013..1499459	+	447	WP_198967264.1	SRPBCC family protein	Toxin
TM48_RS07115 (TM48_01408)	1499571..1500725	-	1155	WP_259594279.1	EspA/EspE family type VII secretion system effector	-
TM48_RS07120 (TM48_01409)	1500990..1501763	+	774	WP_198967265.1	VOC family protein	-
TM48_RS07125 (TM48_01410)	1501834..1502268	+	435	WP_012396159.1	hypothetical protein	-
TM48_RS07130 (TM48_01411)	1502274..1502747	-	474	WP_198967266.1	VOC family protein	-
TM48_RS07135 (TM48_01412)	1502764..1504284	-	1521	WP_198967267.1	carotenoid oxygenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 149 a.a.
• Molecular weight: 15841.35 Da
• Isoelectric Point: 8.6574

>T61847 WP_198967264.1 NZ_CP014860:1499013-1499459 [Mycobacterium shottsii]
MAKLSGSIDVPLSPDEAWMHASDLSRFKEWLTIHRVWRSTLSETLEKGAVVESIVQVKGMHNRIKWTIVRYQPPEGMTLN
GDGVGGVKVKLMAKVAAGEEGSVVSFDVHLGGPALFGPIGMVVAAALRTDINASLRNFVTVFAPSAAG

Nucleotide

• Length: 447 bp

>T61847 NZ_CP014860:1499013-1499459 [Mycobacterium shottsii]
ATGGCGAAACTTTCCGGGTCGATCGACGTGCCGCTGTCTCCGGACGAGGCATGGATGCACGCATCCGATCTCTCCCGCTT
CAAGGAGTGGCTGACGATCCACCGAGTGTGGCGCAGCACGTTGTCCGAAACCCTCGAAAAGGGTGCGGTCGTCGAATCAA
TCGTGCAGGTCAAGGGCATGCACAACCGCATCAAATGGACCATCGTGCGCTACCAGCCGCCGGAAGGCATGACCCTCAAC
GGTGACGGGGTCGGCGGCGTCAAGGTCAAGCTGATGGCCAAGGTTGCAGCCGGAGAAGAAGGCTCGGTCGTCAGCTTCGA
CGTGCACCTTGGTGGGCCGGCTCTGTTCGGTCCGATCGGTATGGTCGTCGCCGCGGCGCTGCGCACCGATATCAACGCAT
CGCTGCGGAACTTCGTCACCGTATTCGCCCCGTCGGCCGCCGGCTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6586.35 Da
• Isoelectric Point: 4.7205

>AT61847 WP_011738542.1 NZ_CP014860:1498820-1499008 [Mycobacterium shottsii]
MGILDKAKDLLAQNADKVETVIDKAGEFVDEKTEGKYSDTIHKVQEGAKKVAGPGAAGEQQN

Nucleotide

• Length: 189 bp

>AT61847 NZ_CP014860:1498820-1499008 [Mycobacterium shottsii]
ATGGGAATCCTGGACAAGGCGAAAGACCTGCTGGCGCAGAACGCCGACAAGGTCGAGACGGTCATCGACAAGGCCGGCGA
ATTCGTCGACGAGAAGACCGAGGGGAAGTACTCCGACACCATCCACAAGGTGCAAGAGGGGGCCAAGAAAGTCGCTGGTC
CCGGCGCCGCGGGCGAGCAGCAGAACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7I7LB02